Author(s):
Adur, Javier Fernando ; Pelegati, Vitor Bianchin, 1982- ; Thomaz, André Alexandre de, 1980- ; Almeida, Diogo Burigo, 1983- ; Andrade, Liliana Aparecida Lucci de Angelo, 1951- ; Böttcher-Luiz, Fátima Aparecida, 1957- ; Carvalho, Hernandes Faustino de, 1965- ; César, Carlos Lenz, 1955-
Date: 2012
Persistent ID: https://hdl.handle.net/20.500.12733/1666702
Origin: Oasisbr
Subject(s): Microscopia de geração de segundo harmônico; Processos multifotônicos; Microscopia óptica não linear; Second harmonic generation microscopy; Multiphoton processes; Nonlinear optical microscopy; Artigo de pesquisa; Microscopia de geração de segundo harmônico; Microscopia de geração de segundo harmônico; Processos multifotônicos; Processos multifotônicos; Microscopia óptica não linear; Microscopia óptica não linear; Second harmonic generation microscopy; Second harmonic generation microscopy; Multiphoton processes; Multiphoton processes; Nonlinear optical microscopy; Nonlinear optical microscopy; Artigo de pesquisa; Artigo de pesquisa
Description
Agradecimentos: This study was supported by the Fundação do Amparo à Pesquisa do Estado de São Paulo - FAPESP (www.fapesp.br). FAPESP Process No 2010/12565-4. FAPESP-INCT-INFABIC Process No 2008/57906-3. The funding institution had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
Abstract: Nonlinear optical (NLO) microscopy techniques have potential to improve the early detection of epithelial ovarian cancer. In this study we showed that multimodal NLO microscopies, including two-photon excitation fluorescence (TPEF), second-harmonic generation (SHG), third-harmonic generation (THG) and fluorescence lifetime imaging microscopy (FLIM) can detect morphological and metabolic changes associated with ovarian cancer progression. We obtained strong TPEF + SHG + THG signals from fixed samples stained with Hematoxylin & Eosin (H&E) and robust FLIM signal from fixed unstained samples. Particularly, we imaged 34 ovarian biopsies from different patients (median age, 49 years) including 5 normal ovarian tissue, 18 serous tumors and 11 mucinous tumors with the multimodal NLO platform developed in our laboratory. We have been able to distinguish adenomas, borderline, and adenocarcinomas specimens. Using a complete set of scoring methods we found significant differences in the content, distribution and organization of collagen fibrils in the stroma as well as in the morphology and fluorescence lifetime from epithelial ovarian cells. NLO microscopes provide complementary information about tissue microstructure, showing distinctive patterns for serous and mucinous ovarian tumors. The results provide a basis to interpret future NLO images of ovarian tissue and lay the foundation for future in vivo optical evaluation of premature ovarian lesions
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
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