Author(s):
Adur, Javier Fernando ; Pelegati, Vitor Bianchin, 1982- ; Thomaz, André Alexandre de, 1980- ; D'Souza Li, Lília Freire Rodrigues, 1967- ; Böttcher-Luiz, Fátima Aparecida, 1957- ; Andrade, Liliana Aparecida Lucci de Angelo, 1951- ; César, Carlos Lenz, 1955-
Date: 2012
Persistent ID: https://hdl.handle.net/20.500.12733/1666725
Origin: Oasisbr
Subject(s): Microscopia óptica não linear; Mamas - Câncer; Neoplasias ovarianas; Osteogenese imperfeita; Matriz extracelular; Colágeno; Nonlinear optical microscopy; Breast - Cancer; Ovarian neoplasms; Osteogenesis imperfecta; Extracellular matrix; Collagen; Osteogenesis imperfecta; Artigo original; Microscopia óptica não linear; Microscopia óptica não linear; Mamas - Câncer; Mamas - Câncer; Neoplasias ovarianas; Neoplasias ovarianas; Osteogenese imperfeita; Osteogenese imperfeita; Matriz extracelular; Matriz extracelular; Colágeno; Colágeno; Nonlinear optical microscopy; Nonlinear optical microscopy; Breast - Cancer; Breast - Cancer; Ovarian neoplasms; Ovarian neoplasms; Osteogenesis imperfecta; Osteogenesis imperfecta; Extracellular matrix; Extracellular matrix; Collagen; Collagen; Osteogenesis imperfecta; Osteogenesis imperfecta; Artigo original; Artigo original
Description
Agradecimentos: The authors are grateful to FAPESP. This work is also linked with the CEPOF (Optics and Photonics Research Center, FAPESP) and National Institute of Photonics Applied to Cell Biology (INFABIC)
Abstract: We show that combined multimodal nonlinear optical (NLO) microscopies, including two-photon excitation fluorescence, second-harmonic generation (SHG), third harmonic generation, and fluorescence lifetime imaging microscopy (FLIM) can be used to detect morphological and metabolic changes associated with stroma and epithelial transformation during the progression of cancer and osteogenesis imperfecta (01) disease. NLO microscopes provide complementary information about tissue microstructure, showing distinctive patterns for different types of human breast cancer, mucinous ovarian tumors, and skin dermis of patients with OI. Using a set of scoring methods (anisotropy, correlation, uniformity, entropy, and lifetime components), we found significant differences in the content, distribution and organization of collagen fibrils in the stroma of breast and ovary as well as in the dermis of skin. We suggest that our results provide a framework for using NLO techniques as a clinical diagnostic tool for human cancer and OI. We further suggest that the SHG and FLIM metrics described could be applied to other connective or epithelial tissue disorders that are characterized by abnormal cells proliferation and collagen assembly
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
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