Document details

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2020-06-23T14:42:33Z No. of bitstreams: 1 2012 - Paula Suzana Elisa Maciel Poll.pdf: 621466 bytes, checksum: e4d0a607f9bfd016da01ca194ad52074 (MD5)

Made available in DSpace on 2020-06-23T14:42:33Z (GMT). No. of bitstreams: 1 2012 - Paula Suzana Elisa Maciel Poll.pdf: 621466 bytes, checksum: e4d0a607f9bfd016da01ca194ad52074 (MD5) Previous issue date: 2012-09-04

Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico, CNPq, Brasil

Considering the complexity of related factors to milk quality and mastitis control, it was aimed to evaluate the dynamic of breast infection and the sensitivity profile of the isolated agents from refrigerated milk in relation to mastitis index and their etiology on six unites of Milk Production (UMP) with high level of Somatic Cell Counts (SCC). During three visits between a 20 days interval were conducted an assessment of clinic mastitis cases and milk samples of those infections were collected to isolation, identification and sensitivity trials. The totalities of each herd were represented by some samples of milk cooling tanks to different analysis: milk composition, somatic cell counts, total bacterial count, antibiotic residues, stability to ethanol, isolation microbial agents? identification and in vitro susceptibility. Between all the proprieties studied the incidence of the mastitis clinic was 0 to 24%, whereas the prevalence was 2,9% to 36%. Among 83 mastitis mammary quarters were isolate 107 bacterial agents in 74 samples and the isolation of fungus came from 27 samples. The main bacterial agents isolated were Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli. Others bacterial also showed a significant isolates were Klebsiella spp. and Pseudomonas spp. The bacterial agents isolated from milk cooling tanks were consistent with the bacterial agents isolated from mastitis cases. However it was a soft divergence between the sensitivity of the milk from affected cows and the milk cooling tanks. Antimicrobial with major in vitro susceptibility from bacterial agents from mastitis cows was enrofloxacin and doxycycline (100%), followed by cefalotin (99,1%); and the isolation from the tanks were: danofloxacin (100%), enrofloxacin (85,2%) and gentamicin (74,1%). The Formulations with higher resistance front to isolated from the mastitis cases was penicillin G associated with novobiocin (53,3%), amoxicillin (49,5%) and ampicillin(42,1%) and compared to isolated from tanks the higher resistances were ampicillin (74,1%), oxacillin (74,1%) and amoxicillin (66,7%). Presence of multiples resistances could be related to indiscriminate use of antibiotics for the treatment of various diseases including mastitis.

Considerando a complexidade de fatores relacionados ? qualidade do leite e controle da mastite, objetivou-se avaliar a din?mica da infec??o mam?ria e o perfil de sensibilidade dos agentes isolados do leite refrigerado em rela??o aos ?ndices de mastite e sua etiologia em seis unidades de produ??o leiteira (UPL) com altas contagens de c?lulas som?ticas (CCS). Em tr?s visitas com intervalos de 20 dias foi realizado um levantamento dos casos de mastite e amostras de leite de quartos mam?rios afetados com mastite cl?nica foram destinadas ao isolamento, identifica??o e testes de sensibilidade. Amostras de leite do conjunto coletadas do tanque de expans?o foram avaliadas quanto ? composi??o, CCS, contagem bacteriana total (CBT), res?duos de antibi?ticos, estabilidade ao etanol, isolamento e identifica??o de agentes microbianos e sensibilidade in vitro. Entre as propriedades avaliadas a incid?ncia de mastite cl?nica variou entre 0 e 24%, enquanto que a preval?ncia oscilou entre 2,9% e 36%. A partir de 83 quartos mam?rios acometidos isolaram-se 107 agentes bacterianos em 74 amostras. Fungos foram isolados de 27 amostras. Os principais agentes bacterianos isolados de amostras de leite de vacas com mastite foram Staphylococcus aureus, Streptococcus agalactiae e Escherichia coli. Tamb?m foram relevantes os isolamentos de Klebsiella spp. e Pseudomonas spp. Estes agentes bacterianos isolados, tanto dos tanques de refrigera??o quanto dos casos de mastite, foram condizentes entre si. No entanto houve uma moderada diverg?ncia entre a sensibilidade in vitro de amostras isoladas de leite de vacas com mastite cl?nica e do leite do tanque. Os antimicrobianos que apresentaram melhor resultado quanto ? sensibilidade dos casos de mastite foram: enrofloxacina e doxiciclina (100%), seguidos da cefalotina (99,1%); e os isolados do tanque apresentaram: danofloxacina (100%), enrofloxacina (85,2%) e gentamicina (74,1%). As formula??es com maior resist?ncia frente aos isolados dos casos de mastite foram penicilina G associada ? novobiocina (53,3%), amoxicilina (49,5%) e ampicilina (42,1%); e frente aos isolados do tanque as maiores resist?ncias foram observadas para ampicilina (74,1%), oxacilina (74,1%) e amoxicilina (66,7%). A presen?a de m?ltiplas resist?ncias pode estar relacionada ao uso indiscriminado de antibi?ticos para tratamento de enfermidades diversas, incluindo a mastite