Author(s):
Sandoval, Carmen ; Araujo, Gabriela ; Sosa, Wilfredo ; Avalos, Sara ; Silveira, Fernando ; Corbett, Carlos ; Z?niga, Concepci?n ; Laurenti, Marcia
Date: 2021
Origin: Oasisbr
Subject(s): Leishmaniose Cut?nea / patologia; Leishmania infantum / parasitologia; Desenluvamentos Cut?neos; Imuno-Histoqu?mica; Macr?fagos; Honduras; Leishmaniose Cut?nea / patologia; Leishmaniose Cut?nea / patologia; Leishmania infantum / parasitologia; Leishmania infantum / parasitologia; Desenluvamentos Cut?neos; Desenluvamentos Cut?neos; Imuno-Histoqu?mica; Imuno-Histoqu?mica; Macr?fagos; Macr?fagos; Honduras; Honduras
Description
The present research was supported by the S?o Paulo Research Foundation (FAPESP) grants #2014/50315-0, #2015/01154-7, #2017/24834-9, #2018/04698-6, Direcci?n de Investigacion y Posgrados de la UNAH grant #02?2015. GA is the recipiente of a scholarship from CAPES (Social Demand) and Laboratorio de Patologia de Molestias Infecciosas (LIM50 HC-FMUSP). ML is the recipient of a research fellowship from the National Council for Scientific and Technological Development (CNPq), grant # 302174/2017-6.
University of S?o Paulo. Department of Pathology, Medical School. Laboratory of Infectious Diseases Pathology. S?o Paulo, SP, Brazil.
University of S?o Paulo. Department of Pathology, Medical School. Laboratory of Infectious Diseases Pathology. S?o Paulo, SP, Brazil.
University of S?o Paulo. Department of Pathology, Medical School. Laboratory of Infectious Diseases Pathology. S?o Paulo, SP, Brazil / National Autonomous University of Honduras. Microbiology Research Institute. Tegucigalpa, Honduras.
National Autonomous University of Honduras. School of Microbiology. Master Program in Infectious and Zoonotic diseases. Tegucigalpa, Honduras.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Federal University of Par?. Institute of Tropical Medicine. Bel?m, PA, Brazil.
University of S?o Paulo. Department of Pathology, Medical School. Laboratory of Infectious Diseases Pathology. S?o Paulo, SP, Brazil.
School Hospital. Department of Health Surveillance. Tegucigalpa, Honduras.
University of S?o Paulo. Department of Pathology, Medical School. Laboratory of Infectious Diseases Pathology. S?o Paulo, SP, Brazil.
Background: Skin lesions of patients affected by non-ulcerated cutaneous leishmaniasis (NUCL) caused by L. (L.) infantum chagasi are characterized by lymphohistiocytic inflammatory infiltrate associated with epithelioid granuloma and scarce parasitism. However, the in situ cellular immune response of these patients is unclear. Therefore, the aim of the present study was to characterize the cellular immune response in the skin lesions of patients affected by NUCL. Methods: Twenty biopsies were processed by immunohistochemistry using primary antibodies to T lymphocytes (CD4, CD8), NK cells, B lymphocytes, macrophages, nitric oxide synthase and interferon-gamma. Results: Immunohistochemistry revealed higher expression of all cellular types and molecules (IFN-?, iNOS) in the dermis of diseased skin compared to the skin of healthy individuals (p < 0.05). Morphometric analysis performed in the skin lesions sections showed the predominance of CD8+ T lymphocytes in the mononuclear infiltrate, followed by macrophages, mostly iNOS+, a response that could be mediated by IFN-?. Conclusion: Our study improves knowledge of the cellular immune response in non-ulcerated or atypical cutaneous leishmaniasis caused by L. (L.) infantum chagasi in Central America and pointed to the pivotal participation of CD8+ T lymphocytes in the host defense mechanisms against the parasite in patients with NUCL.
