Autor(es): Rosa, Carlos Eduardo da ; Figueiredo, Márcio de Azevedo ; Lanes, Carlos Frederico Ceccon ; Almeida, Daniela Volcan ; Marins, Luis Fernando Fernandes
Data: 2012
Origem: Oasisbr
Assunto(s): Antioxidant defense system; Gene expression; Growth hormone; RT–PCR; Transgenic zebrafish; Antioxidant defense system; Antioxidant defense system; Gene expression; Gene expression; Growth hormone; Growth hormone; RT–PCR; RT–PCR; Transgenic zebrafish; Transgenic zebrafish
Submitted by Cristina Damasceno (damascenocris2011@gmail.com) on 2012-12-02T19:33:01Z No. of bitstreams: 1 Genotype-dependent gene expression profile of the antioxidant defense system (ADS) in the liver of a GH-transgenic zebrafish model.pdf: 220200 bytes, checksum: c7becee9bcccfab1508cdff657da5686 (MD5)
Approved for entry into archive by Gabriela Silva da Rosa(gabrielasilvadarosa@gmail.com) on 2012-12-13T22:14:13Z (GMT) No. of bitstreams: 1 Genotype-dependent gene expression profile of the antioxidant defense system (ADS) in the liver of a GH-transgenic zebrafish model.pdf: 220200 bytes, checksum: c7becee9bcccfab1508cdff657da5686 (MD5)
Made available in DSpace on 2012-12-13T22:14:13Z (GMT). No. of bitstreams: 1 Genotype-dependent gene expression profile of the antioxidant defense system (ADS) in the liver of a GH-transgenic zebrafish model.pdf: 220200 bytes, checksum: c7becee9bcccfab1508cdff657da5686 (MD5) Previous issue date: 2011
The aim of this study was to evaluate the effects of growth hormone (GH)overexpression on the gene expression profile of multiple components of the antioxidant defense system(ADS)of different genotypes of a GH transgenic zebrafish (Danio rerio)model. Several ADS-related genes were analyzed by semiquantitative reverse transcription–PCR in the liver of hemizygous (HE) and homozygous (HO)transgenic zebrafish. The results showed a significant reduction in the glutamate cysteine ligase catalytic subunit (GCLC) and the gene expression of two glutathione S-transferase (GST) isoforms and an increase in the glutathione reductase gene in the HO group compared to non-transgenic controls. The expression of the Cu, Zn-superoxide dismutase (SOD1) and catalase (CAT) genes was reduced in HO and HE groups, respectively. Among the ten genes analyzed, two were altered in HE transgenic zebrafish and five were altered in HO transgenic zebrafish. These findings indicate a genotypedependent gene expression profile of the ADS-related genes in the liver of our GH-transgenic zebrafish model and are in agreement with the general effects of GH hypersecretion in the fish and mouse,which involves a reduction in the capability of the tissues to deal with oxidative stress situations. The GH-transgenic zebrafish model used here seems to be an interesting tool for analyzing the effect of different GH expression levels on physiological processes.
