Author(s):
Stein, Fabiana Lorea Paganini ; Schmidt, Bruno Mariano da Silva ; Furlong, Eliana Badiale ; Soares, Leonor Almeida de Souza ; Soares, Maria Cristina Flores ; Vaz, Marta Regina Cezar ; Baisch, Ana Luiza Muccillo
Date: 2011
Origin: Oasisbr
Subject(s): Mesenteric arterial bed; Flavonoids; Experimental hypercholesterolemia; Endothelium; Ilex paraguariensis; Basic science; Nursing; Mesenteric arterial bed; Mesenteric arterial bed; Flavonoids; Flavonoids; Experimental hypercholesterolemia; Experimental hypercholesterolemia; Endothelium; Endothelium; Ilex paraguariensis; Ilex paraguariensis; Basic science; Basic science; Nursing; Nursing
Description
Submitted by Elisangela Pires (emotapires@gmail.com) on 2011-03-23T01:30:37Z No. of bitstreams: 1 Vascular Responses to Extractable Fractions of Ilex paraguariensis in Rats Fed Standard and High-Cholesterol Diets..pdf: 146093 bytes, checksum: cc584df4636bf59ad03b0c8e2a987240 (MD5)
Approved for entry into archive by Barbara Milbrath(barbaramilbrath@yahoo.com.br) on 2011-03-23T17:33:21Z (GMT) No. of bitstreams: 1 Vascular Responses to Extractable Fractions of Ilex paraguariensis in Rats Fed Standard and High-Cholesterol Diets..pdf: 146093 bytes, checksum: cc584df4636bf59ad03b0c8e2a987240 (MD5)
Made available in DSpace on 2011-03-23T17:33:21Z (GMT). No. of bitstreams: 1 Vascular Responses to Extractable Fractions of Ilex paraguariensis in Rats Fed Standard and High-Cholesterol Diets..pdf: 146093 bytes, checksum: cc584df4636bf59ad03b0c8e2a987240 (MD5) Previous issue date: 2005
The authors investigated the vasorelaxant properties of the aqueous (Aq-EF) and acid n-butanolic (acn- BuOH) extractable fractions from Ilex paraguariensis leaves. Perfusion pressure was evaluated using isolated and perfused mesenteric arterial beds (MABs) from rats fed hypercholesterolemic and standard diets. Extract-induced vasorelaxation in the presence and absence of various inhibitors was examined following precontraction of the MABs wi th methoxamine (30 M) solution. In hypercholesterolemic-diet rats, relaxation in intact MABs was significantly decreased with ac-n-BuOHEF bolus (300, 600, 900 g) in comparison to those in standard-diet rats. After the endothelium was stripped from the MABs, the vascular responses to ac-n-BuOHEF and 900 g bolus of Aq-EF were significantly changed. Treatment of the MABs with an inhibitor of nitric oxide synthase, NG-nitro-L-arginine methyl ester hydrochloride (L-NAME, 10 mM), did not change either ac-n-BuOH-EF- or Aq-EF-induced vasodilation except for the 900 g bolus of Aq-EF. The guanilate cyclase inhibitor methylene blue (100 M) did not affect vasodilation for either fraction in the MABs from the hypercholesterolemic-diet rats. The chronic oral administration of I. paraguariensis extract in hypercholesterolemic-diet rats resulted in a significant reduction in serum levels of cholesterol and triglycerides. These results suggest that I. paraguariensis ac-n-BuOH-EF and Aq-EF induce vasodilation in standard-diet rats in a dose-dependent manner and that the hypercholesterolemic diet substantially reduced the effect of ac-n-BuOH-EF on precontracted MABs.
