Author(s):
Martins, Tiago ; Montiel, Rafael ; Medeiros, Jorge ; Oliveira, Luísa ; Simões, Nelson
Date: 2005
Persistent ID: http://hdl.handle.net/10400.3/1073
Origin: Repositório da Universidade dos Açores
Subject(s): Baculovirus; Polyhedrin; Ultrastructure; Spodoptera littoralis; Spodoptera littoralis nucleopolyhedrosis; Virus characterization; Virulence; Baculovirus; Baculovirus; Polyhedrin; Polyhedrin; Ultrastructure; Ultrastructure; Spodoptera littoralis; Spodoptera littoralis; Spodoptera littoralis nucleopolyhedrosis; Spodoptera littoralis nucleopolyhedrosis; Virus characterization; Virus characterization; Virulence; Virulence
Description
A nucleopolyhedrovirus (SpliMNPV-Az) was isolated from diseased larvae of Spodoptera littoralis, collected at the Island of S. Miguel in Azores. The virulence of this isolate was tested against S. littoralis larvae in laboratory. LD50 against 2nd and 3rd instars were not significantly different larvae, but both were significantly different from that against 4th instar. The complete codons sequence of SpliMNPV-Az Polh gene obtained was 750 bp (NCBI GenBank Accession No. AY600451). This sequence was compared to other 38 polyhedrin genes from NPVs and to 6 granulin genes from GVs and resulted to be identical to the sequence of a SpliMNPV previously published, thus indicating that the natural host of SpliMNPV-Az must be S. littoralis. Genetic distances estimated from restriction enzymes profiles showed SpliMNPV-Az is close to the Egyptian SpliMNPV type B, despite some degree of genetic divergence suggested by slight differences observed on PstI profile.
