Author(s):
Pinheiro, Antonio Luiz Barbosa ; Gerbi, Marleny E. Martinez ; Ponzi, Elizabeth Arruda Carneiro ; Marques, Aparecida M. C. ; Carvalho, Carolina Montagn de ; Santos, Rafael de Carneiro ; Oliveira, Priscila Chagas ; Noia, Manuela Pimentel ; Pinheiro, Antonio Luiz Barbosa ; Gerbi, Marleny E. Martinez ; Ponzi, Elizabeth Arruda Carneiro ; Marques, Aparecida M. C. ; Carvalho, Carolina Montagn de ; Santos, Rafael de Carneiro ; Oliveira, Priscila Chagas ; Noia, Manuela Pimentel
Date: 2014
Origin: Oasisbr
Subject(s): Photobiomodulation; Rats; Morphogenetic proteins; Photobiomodulation; Photobiomodulation; Rats; Rats; Morphogenetic proteins; Morphogenetic proteins
Description
Texto completo: acesso restrito. p. 167-174
Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-12-10T14:26:08Z No. of bitstreams: 1 pho%2E2007%2E2027.pdf: 5679695 bytes, checksum: 270c4e5d8071010e93e341b1c296839b (MD5)
Approved for entry into archive by Flávia Ferreira (flaviaccf@yahoo.com.br) on 2014-02-19T18:33:08Z (GMT) No. of bitstreams: 1 pho%2E2007%2E2027.pdf: 5679695 bytes, checksum: 270c4e5d8071010e93e341b1c296839b (MD5)
Made available in DSpace on 2014-02-19T18:33:08Z (GMT). No. of bitstreams: 1 pho%2E2007%2E2027.pdf: 5679695 bytes, checksum: 270c4e5d8071010e93e341b1c296839b (MD5) Previous issue date: 2008
Objective: This study assessed histologically the effect of laser photobiomodulation on the repair of surgical defects created in the femurs of Wistar rats treated or not treated with bone morphogenetic proteins (BMPs) and organic bovine bone graft. Background Data: This paper is part of an ongoing series of works in which biomaterials and/or guided bone regeneration (GBR) are used in association with laser photobiomodulation. Several previous reports from our group have shown that the use of laser photobiomodulation improves the treatment of bone defects. Materials and Methods: Forty-eight adult male Wistar rats were divided into four randomized groups: group 1 (controls, n = 12); group 2 (laser photobiomodulation, n = 12); group 3 (BMPs + organic bovine bone graft + GBR, n = 12); and group 4 (BMPs + organic bovine bone graft + GBR + laser photobiomodulation, n = 12). The irradiated groups received seven irradiations every 48 h, the first immediately after the surgical procedure. Laser photobiomodulation (830 nm, 40 mW, CW, 0.6 mm) consisted of a total of 16 J/cm2 per session at four points (4 J/cm2 each) equally spaced around the periphery of the defect. The animals were sacrificed after 15, 21, and 30 d, and the specimens were routinely embedded in wax and stained with hematoxylin and eosin and Sirius red stains and analyzed under light microscopy. Results: The results showed histological evidence of increased deposition of collagen fibers (at 15 and 21 d), as well as an increased amount of well-organized bone trabeculi at the end of the experimental period (30 d) in irradiated animals compared to non-irradiated controls. Conclusion: We concluded that the use of laser photobiomodulation in association with BMPs, organic bovine bone grafts, and GBR increases the positive biomodulating effects of laser energy.
