Author(s):
Sara Santos de Carvalho ; Ana Cristina Simões e Silva ; Adriano de Paula Sabino ; Fernanda Cristina Gontijo Evangelista ; Karina Braga Gomes ; Luci Maria SantAna Dusse ; Danyelle Romana Alves Rios
Date: 2022
Persistent ID: http://hdl.handle.net/1843/39901
Origin: Oasisbr
Subject(s): Diálise; Plasma; Doenças cardiovasculares; Insuficiência renal crônica; Reação em cadeia da polimerase; Genótipo; Alelos; Diálise; Diálise; Plasma; Plasma; Doenças cardiovasculares; Doenças cardiovasculares; Insuficiência renal crônica; Insuficiência renal crônica; Reação em cadeia da polimerase; Reação em cadeia da polimerase; Genótipo; Genótipo; Alelos; Alelos
Description
CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
FAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
Background: There is substantial evidence that chronic renal and cardiovascular diseases are associated with coagulation disorders, endothelial dysfunction, inflammation and fibrosis. Angiotensin-Converting Enzyme Insertion/Deletion polymorphism (ACE I/D polymorphism) has also be linked to cardiovascular diseases. Therefore, this study aimed to compare plasma levels of ultrassensible C-reactive protein (usCRP), PAI-1, D-dimer and TGF-β1 in patients undergoing HD with different ACE I/D polymorphisms. Methods: The study was performed in 138 patients at ESRD under hemodialysis therapy for more than six months. The patients were divided into three groups according to the genotype. Genomic DNA was extracted from blood cells (leukocytes). ACE I/D polymorphism was investigated by single polymerase chain reaction (PCR). Plasma levels of D-dimer, PAI-1 and TGF-β1 were measured by enzyme-linked immunosorbent assay (ELISA), and the determination of plasma levels of usCRP was performed by immunonephelometry. Data were analyzed by the software SigmaStat 2.03. Results: Clinical characteristics were similar in patients with these three ACE I/D polymorphisms, except for interdialytic weight gain. I allele could be associated with higher interdialytic weight gain (P =0.017). Patients genotypedas DDandasIDhadsignificantly higher levels of PAI-1 than those with II genotype. Other laboratory parameters did not significantly differ among the three subgroups (P = 0.033). Despite not reaching statistical significance, plasma levels of usCRPwerehigherinpatients carrying the D allele. Conclusion: ACEI/Dpolymorphisms could beassociated with changes in the regulation of sodium, fibrinolytic system, and possibly, inflammation. Our data showed that high levels of PAI-1 are detected when Dallele is present, whereas greater interdialytic gain is associated with the presence of I allele. However, further studies with different experimental designs are necessary to elucidate the mechanisms involved in these associations.
