Autor(es): Sousa, Isabel Garcia ; Almo, Manuela Maragno do ; Simi, Kelly Cristina Rodrigues ; Bezerra, Maryani Andressa Gomes ; Andrade, Rosângela Vieira ; Maranhão, Andréa Queiroz ; Brígido, Marcelo de Macedo
Data: 2017
Origem: Oasisbr
Assunto(s): Anticorpos; Imunologia; Anticorpos; Anticorpos; Imunologia; Imunologia
Background: Anti-CD3 therapy can induce immunosuppression by several non mutually exclusive mechanisms that have been proposed to explain the therapeutic effect the administration anti-CD3 mAb, but its immunoregulatory mechanism is still not completely clear. In T cells, microRNAs (miRNAs) regulate several pathways, including those associated with immune tolerance. Here, we report changes in miRNA expression in T cells following treatment with anti-human CD3 antibodies. Peripheral blood mononuclear cells were cultured in the presence of the monoclonal antibody OKT3 or a recombinant fragment of humanized anti-CD3. Following these treatments, the expression profiles of 31 miRNA species were assessed in T cells using TaqMan arrays. Results: Eight of the tested miRNAs (miR-155, miR-21, miR-146a, miR-210, miR-17, miR-590-5p, miR-106b and miR- 301a) were statistically significantly up- or down-regulated relative to untreated cells. Conclusions: Stimulation of T cells with anti-human CD3 antibodies alters miRNA expression patterns, including of miRNA species associated with immune regulatory pathways.
