Author(s): Sato, Vanessa Sayuri [UNESP] ; Jorge, Joao Atilio ; Oliveira, Wanderley Pereira ; Fernandes Souza, Claudia Regina ; Souza Guimaraes, Luis Henrique [UNESP]
Date: 2014
Persistent ID: http://hdl.handle.net/11449/111784
Origin: Oasisbr
Subject(s): Rhizopus micros porus; spray drying; phytase; enzyme stabilization; Rhizopus micros porus; Rhizopus micros porus; spray drying; spray drying; phytase; phytase; enzyme stabilization; enzyme stabilization
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Microbial phytases are enzymes with biotechnological interest for the feed industry. In this article, the effect of spray-drying conditions on the stability and activity of extracellular phytase produced by R. microsporus var. micros porus biofilm is described. The phytase was spray-dried in the presence of starch, corn meal (>150 mu m), soy bean meal (SB), corn meal (<150 mu m) (CM), and maltodextrin as drying adjuvants. The residual enzyme activity after drying ranged from 10.7% to 60.4%, with SB and CM standing out as stabilizing agents. Water concentration and residual enzyme activity were determined in obtained powders as a function of the drying condition. When exposed to different pH values, the SB and CM products were stable, with residual activity above 50% in the pH range from 4.5 to 8.5 for 60 min. The use of CM as drying adjuvant promoted the best retention of enzymatic activity compared with SB. Spray drying of the R. micros porus var. micros porus phytase using different drying adjuvants showed interesting results, being quite feasible with regards their biotechnological applications, especially for poultry diets.
Univ Estadual Paulista, Inst Chem Araraquara, BR-14800900 Sao Paulo, Brazil
Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, BR-14040901 Sao Paulo, Brazil
Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, BR-14040900 Sao Paulo, Brazil
Univ Estadual Paulista, Inst Chem Araraquara, BR-14800900 Sao Paulo, Brazil
