Autor(es):
Ronchi, Carlos Fernando [UNESP] ; Fioretto, José Roberto [UNESP] ; Ferreira, Ana Lúcia dos Anjos [UNESP] ; Berchieri-Ronchi, Carolina Bragiola [UNESP] ; Corrêa, Camila Renata [UNESP] ; Kurokawa, Cilmery Suemi [UNESP] ; Carpi, Mario Ferreira [UNESP] ; Moraes, Marcos Aurelio [UNESP] ; Yeum, Kyung-Jin
Data: 2014
Identificador Persistente: http://hdl.handle.net/11449/13174
Origem: Oasisbr
Assunto(s): comet assay; DNA damage; antioxidant performance; total antioxidant performance assay; mechanical ventilation; comet assay; comet assay; DNA damage; DNA damage; antioxidant performance; antioxidant performance; total antioxidant performance assay; total antioxidant performance assay; mechanical ventilation; mechanical ventilation
Descrição
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
USDA Agricultural Research Service
Ronchi CF, Fioretto JR, Ferreira ALA, Berchieri-Ronchi CB, Correa CR, Kurokawa CS, Carpi MF, Moraes MA, Yeum K-J. Biomarkers for oxidative stress in acute lung injury induced in rabbits submitted to different strategies of mechanical ventilation. J Appl Physiol 112: 1184-1190, 2012. First published February 2, 2012; doi:10.1152/japplphysiol.01334.2011.-Oxidative damage has been said to play an important role in pulmonary injury, which is associated with the development and progression of acute respiratory distress syndrome (ARDS). We aimed to identify biomarkers to determine the oxidative stress in an animal model of acute lung injury (ALI) using two different strategies of mechanical ventilation. Rabbits were ventilated using either conventional mechanical ventilation (CMV) or high-frequency oscillatory ventilation (HFOV). Lung injury was induced by tracheal saline infusion (30 ml/kg, 38 degrees C). In addition, five healthy rabbits were studied for oxidative stress. Isolated lymphocytes from peripheral blood and lung tissue samples were analyzed by alkaline single cell gel electrophoresis (comet assay) to determine DNA damage. Total antioxidant performance (TAP) assay was applied to measure overall antioxidant performance in plasma and lung tissue. HFOV rabbits had similar results to healthy animals, showing significantly higher antioxidant performance and lower DNA damage compared with CMV in lung tissue and plasma. Total antioxidant performance showed a significant positive correlation (r = 0.58; P = 0.0006) in plasma and lung tissue. In addition, comet assay presented a significant positive correlation (r = 0.66; P = 0.007) between cells recovered from target tissue and peripheral blood. Moreover, antioxidant performance was significantly and negatively correlated with DNA damage (r = -0.50; P = 0.002) in lung tissue. This study indicates that both TAP and comet assay identify increased oxidative stress in CMV rabbits compared with HFOV. Antioxidant performance analyzed by TAP and oxidative DNA damage by comet assay, both in plasma, reflects oxidative stress in the target tissue, which warrants further studies in humans.
Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA
São Paulo State Univ UNESP, Dept Pediat, Botucatu Med Sch, Botucatu, SP, Brazil
São Paulo State Univ UNESP, Dept Internal Med, Botucatu Med Sch, Botucatu, SP, Brazil
São Paulo State Univ UNESP, Dept Pediat, Botucatu Med Sch, Botucatu, SP, Brazil
São Paulo State Univ UNESP, Dept Internal Med, Botucatu Med Sch, Botucatu, SP, Brazil
FAPESP: 08/08199-2
USDA: 1950-51000-065-08S
