Author(s): Valera, Larissa Serrani [UNESP] ; Jorge, Joao Atilio ; Souza Guimaraes, Luis Henrique
Date: 2018
Persistent ID: http://hdl.handle.net/11449/158592
Origin: Oasisbr
Subject(s): Aspergillus; Microbial enzymes; Solid-state fermentation; Tannase; Tannic acid; Aspergillus; Aspergillus; Microbial enzymes; Microbial enzymes; Solid-state fermentation; Solid-state fermentation; Tannase; Tannase; Tannic acid; Tannic acid
Made available in DSpace on 2018-11-26T15:28:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-11-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Background: Tannases are enzymes with biotechnological potential produced mainly by microorganisms as filamentous fungi. In this context, the production and characterization of a multi-tolerant tannase from Aspergillus carbonarius is described. Results: The filamentous fungus A. carbonarius produced high levels of tannase when cultivated under solid-state fermentation using green tea leaves as substrate/carbon source and tapwater at a 1:1 ratio as the moisture agent for 72 h at 30 degrees C. Two tannase activity peakswere obtained during the purification step using DEAE-Cellulose. The second peak (peak II) was purified 11-fold with 14% recovery from a Sepharose CL-6B chromatographic column. The tannase from peak II (tannase II) was characterized as a heterodimeric glycoprotein of 134.89 kDa, estimated through gel filtration, with subunits of 65 kDa and 100 kDa, estimated through SDS-PAGE, and 48% carbohydrate content. The optimal temperature and pH for tannase II activity was 60 degrees C and 5.0, respectively. The enzyme was fully stable at temperatures ranging from 20-60 degrees C for 120 min, and the half-life (T1/2) at 75 degrees C was 62 min. The activation energy was 28.93 kJ/mol. After incubation at pH 5.0 for 60 min, 75% of the enzyme activity was maintained. However, enzyme activity was increased in the presence of AgNO3 and it was tolerant to solvents and detergents. Tannase II exhibited a better affinity for methyl gallate (Km = 1.42 mM) rather than for tannic acid (Km = 2.2 mM). Conclusion: A. carbonarius tannase presented interesting properties as, for example, multi-tolerance, which highlight its potential for future application. (C) 2015 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved.
Univ Estadual Paulista, Inst Quim Araraquara, BR-14800900 Sao Paulo, Brazil
Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Pret, Dept Biol, BR-14040901 Sao Paulo, Brazil
Univ Estadual Paulista, Inst Quim Araraquara, BR-14800900 Sao Paulo, Brazil
FAPESP: 2011/50880-1
