Author(s):
Guerino, Laura Rocha [UNESP] ; Pecora, Iracy Lea [UNESP] ; Miranda, Marcel Sabino ; Aguiar-Silva, Cryslaine ; Carvalho, Omar dos Santos ; Caldeira, Roberta Lima ; Silva, Reinaldo Jose da [UNESP]
Date: 2018
Persistent ID: http://hdl.handle.net/11449/162578
Origin: Oasisbr
Subject(s): Rat lungworm; Giant African snail; Eosinophilic meningitis; Nematode; Emerging parasitosis; Rat lungworm; Rat lungworm; Giant African snail; Giant African snail; Eosinophilic meningitis; Eosinophilic meningitis; Nematode; Nematode; Emerging parasitosis; Emerging parasitosis
Description
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Introduction: Angiostrongylus cantonensis causes eosinophilic meningoencephalitis in humans. Worldwide expansion of this nematode is linked to the dispersion of their hosts. This study aimed to determine the prevalence of A. cantonensis infection in Achatina fulica in the nine municipalities that make up Baixada Santista, Sao Paulo, Brazil. Methods: Angiostrongylus cantonensis larvae were analyzed using optical microscopy. We performed polymerase chain reaction and restriction fragment length polymorphism using restriction endonuclease ClaI, directed to the internal transcribed spacer region 2 of A. cantonensis larval DNA. Results: Of the 540 snails analyzed, 117 (21.7%) were infected by A. cantonensis. For morphological and morphometric analyses, 60 larvae were used. Second-stage larvae were, on average, 358.2 mu m long and 26.4 mu m wide, while third-stage larvae were, on average, 450 mu m long and 21.12 mu m wide. The tails of the larvae ended in a fine tip. Conclusions: All municipalities comprising Baixada Santista had A. fulica that were naturally infected with A. cantonensis. All of the observed characteristics were typical of the species.
Univ Estadual Paulista, Lab Parasitol Animais Silvestres, Dept Parasitol, Botucatu, SP, Brazil
Univ Estadual Paulista, Lab Moluscos, Sao Vicente, SP, Brazil
Univ Estadual Campinas, Lab Malacol, Dept Biol Anim, Campinas, SP, Brazil
Fundacao Oswaldo Cruz, Lab Helmintol & Malacol Med, Ctr Pesquisas Rene Rachou, Belo Horizonte, MG, Brazil
Univ Estadual Paulista, Lab Parasitol Animais Silvestres, Dept Parasitol, Botucatu, SP, Brazil
Univ Estadual Paulista, Lab Moluscos, Sao Vicente, SP, Brazil
FAPESP: 2011/05893-8
