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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Exposure to Tetrachlorodibenzo-p-dioxin (TCDD) in male rats promotes, decreased sperm concentration, alterations in motility and in sperm transit time. We evaluated the effect transgenerational of in utero exposure to low doses TCDD in the sperm quality. Pregnant rats (F0) were exposed to 0.1; 0.5 and 1.0 μg of TCDD, on gestational day 15, coincides with the end of most organogenesis in the fetus. Adult male offspring (F1, F2 and F3 generation) were investigated for fertility after artificial insemination in utero. After collection of the uterus and ovaries, the numbers of corpora lutea and implants were determined. TCDD provoked alterations in sperm morphology and diminution in serum testosterone levels and sperm transit time in the cauda epididymis. The fertility significantly decreased in all the generations, at least at one dose. In conclusion, TCDD exposure decreases rat sperm quality and fertility in adult male offspring and this effects persist into the next generation.
Laboratory of Reproductive and Developmental Biology and Toxicology Department of Morphology Institute of Biosciences of Botucatu UNESP—Univ Estadual, Botucatu
Department of Morphology Stomatology and Physiology School of Dentistry USP—University of São Paulo, Ribeirão Preto
Laboratory of Reproductive and Developmental Biology and Toxicology Department of Morphology Institute of Biosciences of Botucatu UNESP—Univ Estadual, Botucatu
FAPESP: 2012/00365-6
