Author(s): Jusi, Márcia Mariza Gomes [UNESP] ; Oliveira, Trícia Maria Ferreira de Sousa ; Nakaghi, Andréa Cristina Higa ; André, Marcos Rogério [UNESP] ; Machado, Rosangela Zacarias [UNESP]
Date: 2018
Persistent ID: http://hdl.handle.net/11449/177514
Origin: Oasisbr
Subject(s): A2; Diagnosis; ELISA; Escherichia coli; Leishmania infantum; A2; A2; Diagnosis; Diagnosis; ELISA; ELISA; Escherichia coli; Escherichia coli; Leishmania infantum; Leishmania infantum
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This study aimed to: express a recombinant A2 family protein of Leishmania chagasi, Jaboticabal strain, test this protein as an antigen in serological assays, and investigate its antigenicity and immunogenicity. A protein coded by an allele of the A2 gene isolated from L. chagasi was expressed in three different strains of Escherichia coli. We used 29 sera samples from Leishmune-vaccinated dogs, 482 sera samples from dogs from endemic areas (positive controls), and 170 sera samples from dogs from non-endemic areas (negative controls) in ELISA tests using soluble Leishmania antigen (SLA) and His-A2 as antigen. Expressed proteins showed, by western blotting, the expression of an 11 KDa protein. Sixtythree percent (303/482) of the samples from endemic areas were positive by ELISA His-A2, whereas 93.1% (27/29) of Leishmune®-vaccinated animals were negative by His-A2-ELISA. Anti-A2 antibodies from mice inoculated with the A2 protein were detected in slides containing amastigote forms, but not in slides containing promastigote forms. The A2 recombinant protein from L. chagasi may be a useful tool in the diagnosis of CVL, and further tests regarding the infection stage and the specie of parasite at which the dogs are sampled should provide a better understanding of our results.
Laboratório de Imunoparasitologia, Departamento de Patologia Veterinária, Universidade Estadual Paulista – UNESP
Departamento de Zootecnia, Universidade de São Paulo – USP
Laboratório de Imunoparasitologia, Departamento de Patologia Veterinária, Universidade Estadual Paulista – UNESP
