Author(s):
Hudari, Felipe Fantinato [UNESP] ; da Silva, Bianca Ferreira [UNESP] ; Pividori, Maria Isabel ; Zanoni, Maria Valnice Boldrin [UNESP]
Date: 2018
Persistent ID: http://hdl.handle.net/11449/177599
Origin: Oasisbr
Subject(s): Diuretic; Graphite-epoxy composite electrode; Magnetic particles; Multivariate optimization; Triamterene; Voltammetric sensor; Diuretic; Diuretic; Graphite-epoxy composite electrode; Graphite-epoxy composite electrode; Magnetic particles; Magnetic particles; Multivariate optimization; Multivariate optimization; Triamterene; Triamterene; Voltammetric sensor; Voltammetric sensor
Description
Made available in DSpace on 2018-12-11T17:26:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-09-01
Some diuretic substances are controlled and monitored by the World Anti-Doping Agency as prohibited substances for use by athletes, such as triamterene (TRT). Thus, this work describes a voltammetric method based on graphite-epoxy composite electrode modified by tosyl-functionalized magnetic particles (GECE/MPs-To) for determination of TRT diuretic in urine sample. The TRT presented an oxidation peak at +1.24 V at GECE/MPs-To with irreversible behavior. Controlled potential electrolysis of the TRT at +1.26 V indicated the two electrons are transferred during amine group oxidation and the main product was identified by LC-MS/MS. The anodic peak current is 25 % higher at the modified electrode, suggesting that TRT is adsorbed on the magnetic particles. Using optimized conditions by using multivariate optimization of the parameters inherent of the square wave voltammetry, a calibration curve was constructed with a linear relationship for TRT from 0.500 to 99.8 μmol L−1. The limits of detection and quantification were 1.47 and 4.91 × 10−7 mol L−1, respectively. The proposed method was applied to urine sample and validated by LC-MS/MS technique where the values found and compared between the two techniques showed no significant difference at 95 % confidence.
Departamento de Química UNESP Universidade Estadual Paulista Júlio Mesquita Filho Instituto de Química de Araraquara, Rua Francisco Degni, 55, Bairro Quitandinha,
Departamento de Química Grupo de Sensor e Biosensor Universidade Autônoma de Barcelona, Bellaterra
Departamento de Química UNESP Universidade Estadual Paulista Júlio Mesquita Filho Instituto de Química de Araraquara, Rua Francisco Degni, 55, Bairro Quitandinha,
