Author(s): Barretto, Adriana Beatriz [UNESP] ; Maia, Caroline Marques [UNESP] ; Alves, Nina Pacheco Capelini [UNESP] ; Giaquinto, Percilia Cardoso [UNESP]
Date: 2018
Persistent ID: http://hdl.handle.net/11449/180092
Origin: Oasisbr
Subject(s): Classical conditioning; Fish learning; Memory; Water jet; Classical conditioning; Classical conditioning; Fish learning; Fish learning; Memory; Memory; Water jet; Water jet
Made available in DSpace on 2018-12-11T17:38:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-10-01
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Classical conditioning in animals is a learning procedure involving a biologically relevant stimulus paired with a previously neutral stimulus. In fish, light and sound are frequently used as previously neutral stimuli for conditioning tests. However, in laboratory experiments with replicates, such stimuli may influence the responses of fish in nearby aquariums. Herein, we developed a simple applicable methodology for classical conditioning in fish that prevents this type of influence. We isolated fish in individual aquariums and introduced a water jet that caused localized water movement, followed by the introduction of a food pellet. These procedures were repeated for each fish for 20 days. After 14 days, all fish were conditioned. Moreover, in subsequent probe trials (memory retention tests) conducted within 32 days after conditioning procedures, fish responded accordingly. These findings corroborate the applicability and usefulness of the method tested herein especially under lab conditions. Therefore, we suggest that a simple water jet is a useful and reliable tool for fish conditioning in future studies.
Department of Physiology Institute of Biosciences (IB) São Paulo State University (UNESP), Street Prof. Dr. Antonio Celso Wagner Zanin, s/n°
Department of Physiology Institute of Biosciences (IB) São Paulo State University (UNESP), Street Prof. Dr. Antonio Celso Wagner Zanin, s/n°
