Autor(es): Barros, Rodrigo G. [UNESP] ; Lima, Paula F. [UNESP] ; Soares, Ana Caroline S. [UNESP] ; Sanches, Lorena [UNESP] ; Price, Christopher A. ; Buratini, Jose [UNESP]
Data: 2019
Identificador Persistente: http://hdl.handle.net/11449/186763
Origem: Oasisbr
Assunto(s): FGF2; IVM; Oocyte-secreted factors; Meiosis; Cumulus expansion; Apoptosis; FGF2; FGF2; IVM; IVM; Oocyte-secreted factors; Oocyte-secreted factors; Meiosis; Meiosis; Cumulus expansion; Cumulus expansion; Apoptosis; Apoptosis
Made available in DSpace on 2019-10-06T02:14:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-05-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
PurposeWe first assessed regulation of FGF2 expression in cumulus cells by FSH and oocyte-secreted factors during in vitro maturation (IVM). Then, we tested the hypothesis that FGF2 regulates meiotic progression, cumulus expansion, and apoptosis in cumulus-oocyte complexes (COC) undergoing IVM.MethodsIn vitro maturation of bovine COC was utilized as a model to assess regulation of FGF2 expression by FSH and oocyte-secreted factors (via microsurgical removal of the oocyte), as well as effects of graded doses of FGF2 on meiotic progression, degree of cumulus expansion, dissociation of cumulus cells, and cumulus cells apoptosis. Expression of genes regulating functional endpoints altered by FGF2 treatment was assessed in cumulus cells by real-time PCR. Cultures were replicated 4-5 times and effects of treatments were tested by ANOVA.ResultsFGF2 mRNA expression was increased by FSH and oocyte-secreted factors during IVM. Addition of FGF2 to the IVM medium advanced meiosis resumption, decreased the ease with which cumulus cells were dissociated, and inhibited cumulus cells apoptosis. Decreased cumulus dissociation was accompanied by decreased expression of TNFAIP6.ConclusionsThis is the first study showing that FGF2 expression is regulated by the oocyte in cumulus cells. Moreover, we report novel effects of FGF2 on cumulus cell survival and extracellular matrix (ECM) quality during IVM that may favor acquisition of developmental competence and suggest physiological roles during the final steps of COC differentiation.
Univ Estadual Paulista, Dept Fisiol, Inst Biociencias, BR-18618970 Sao Paulo, Brazil
Univ Montreal, Fac Med Vet, Ctr Rech Reprod & Fertilite, St Hyacinthe, PQ J2S 7C6, Canada
Univ Estadual Paulista, Dept Fisiol, Inst Biociencias, BR-18618970 Sao Paulo, Brazil
FAPESP: 12/06417-8
CNPq: 312521/2014-6
