Author(s): De Paula, Mirian Michelle Machado ; Bassous, Nicole Joy ; Afewerki, Samson ; Harb, Samarah Vargas [UNESP] ; Ghannadian, Paria ; Marciano, Fernanda Roberta ; Viana, Bartolomeu Cruz ; Tim, Carla Roberta ; Webster, Thomas Jay ; Lobo, Anderson Oliveira
Date: 2019
Persistent ID: http://hdl.handle.net/11449/187192
Origin: Oasisbr
Made available in DSpace on 2019-10-06T15:28:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-12-01
Instituto de Desenvolvimento Sustentável Mamirauá
Herein, we report the design of electrospun ultrathin fibers based on the combination of three different polymers polycaprolactone (PCL), polyethylene glycol (PEG), and gelatin methacryloyl (GelMA), and their potential bactericidal activity against three different bacteria Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aeruginosa), and Methi-cillin-resistant Staphylococcus aureus (MRSA). We evaluated the morphology, chemical structure and wettability before and after UV photocrosslinking of the produced scaffolds. Results showed that the developed scaffolds presented hydrophilic properties after PEG and GelMA incorporation. Moreover, they were able to significantly reduce gram-positive, negative, and MRSA bacteria mainly after UV photocrosslinking (PCL:PEG:GelMa-UV). Furthermore, we performed a series of study for gaining a better mechanistic understanding of the scaffolds bactericidal activity through protein adsorption study and analysis of the reactive oxygen species (ROS) levels. Furthermore, the in vivo subcutaneous implantation performed in rats confirmed the biocompatibility of our designed scaffolds.
Faculty of Medical Sciences State University of Campinas
Department of Chemical Engineering Northeastern University
Department of Chemical Engineering Koch Institute for Integrative Cancer Research Massachusetts Institute of Technology
Division of Gastroenterology Brigham and Women´s Hospital Harvard Medical School
Institute of Chemistry UNESP-São Paulo State University
Institute of Science and Technology Brasil University
LIMAV-Interdisciplinary Laboratory for Advanced Materials PPGCM-Materials Science and Engineering graduate program UFPI-Federal University of Piauí
Department of Physics UFPI-Federal University of Piauí
Department of Chemistry Massachusetts Institute of Technology
Institute of Chemistry UNESP-São Paulo State University
Instituto de Desenvolvimento Sustentável Mamirauá: Serra-1709-19479
