Author(s): Pansani, Taisa Nogueira [UNESP] ; Basso, Fernanda Gonçalves ; Souza, Isabela dos Reis [UNESP] ; Hebling, Josimeri [UNESP] ; de Souza Costa, Carlos Alberto [UNESP]
Date: 2019
Persistent ID: http://hdl.handle.net/11449/188932
Origin: Oasisbr
Subject(s): Abutment; Cell survival; Epidermal growth factor; Fibroblast; Titanium; Abutment; Abutment; Cell survival; Cell survival; Epidermal growth factor; Epidermal growth factor; Fibroblast; Fibroblast; Titanium; Titanium
Made available in DSpace on 2019-10-06T16:23:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-06-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Objectives: Different strategies, such as modifications on the implant abutments surface have been proposed to accelerate and improve the formation of the biological seal (BS). The aim of this study was to characterize a titanium (Ti) surface impregnated with epidermal growth factor (EGF) and to assess its influence on the metabolism and adhesion of oral mucosal cells. Design: Ti discs were coated with EGF (100 nM) conjugated with a fluorophore and analyzed by fluorescence microscopy. The surface roughness analysis (Ra) of the EGF-coated Ti was performed by confocal microscopy. The EGF released in the wet environment was determined at 0, 24, 48 and 72 h by fluorimetric quantification. For assessment of the biological effects of EGF-coated Ti, gingival fibroblasts were seeded (5 × 10 4 cells) onto the substrate coated or not with this growth factor. After 24 h, cell adhesion and viability were evaluated by ANOVA and Tukey tests, α =.05. Results: Immediate release of EGF as well as its incorporation by fibroblasts within 1 h after cells were seeded was observed. EGF-coated Ti discs presented significantly enhance surface roughness. Increased cell viability was observed on the EGF-coated discs. Conclusion: EGF applied to Ti discs stimulated the adhesion and metabolism of gingival fibroblasts and could be considered as an interesting alternative for improving the BS.
Department of Dental Materials and Prosthodontics UNESP – Univ. Estadual Paulista Araraquara School of Dentistry
Department of Dentistry Universidade de Ribeirão Preto UNAERP
Department of Orthodontics and Pediatric Dentistry UNESP – Univ. Estadual Paulista Araraquara School of Dentistry
Department of Physiology and Pathology UNESP – Univ. Estadual Paulista Araraquara School of Dentistry Araraquara
Department of Dental Materials and Prosthodontics UNESP – Univ. Estadual Paulista Araraquara School of Dentistry
Department of Orthodontics and Pediatric Dentistry UNESP – Univ. Estadual Paulista Araraquara School of Dentistry
Department of Physiology and Pathology UNESP – Univ. Estadual Paulista Araraquara School of Dentistry Araraquara
FAPESP: 2015/19364-8
CNPq: 442637/2014-4
