Author(s):
Souza, José Antonio Santos [UNESP] ; Barbosa, Debora Barros [UNESP] ; do Amaral, Jackeline Gallo [UNESP] ; Monteiro, Douglas Roberto ; Gorup, Luiz Fernando ; de Souza Neto, Francisco Nunes ; Fernandes, Renan Aparecido ; Fernandes, Gabriela Lopes [UNESP] ; de Camargo, Emerson Rodrigues ; Agostinho, Alessandra Marçal ; Delbem, Alberto Carlos Botazzo [UNESP]
Date: 2019
Persistent ID: http://hdl.handle.net/11449/190206
Origin: Oasisbr
Subject(s): Biofilms; Candida albicans; Metal nanoparticles; Polyphosphates; Streptococcus mutans; Biofilms; Biofilms; Candida albicans; Candida albicans; Metal nanoparticles; Metal nanoparticles; Polyphosphates; Polyphosphates; Streptococcus mutans; Streptococcus mutans
Description
Made available in DSpace on 2019-10-06T17:05:45Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01
Nanocomposites containing antimicrobial agents and calcium phosphates have been developed. Thus, this study assessed the effects of two compounds containing silver nanoparticles (AgNPs) and β-calcium glycerophosphate (CaGP), associated or not with tyrosol (TYR), against planktonic cells and biofilms of Candida albicans and Streptococcus mutans. The nanocompounds were synthesized through chemical and ‘green’ processes and characterized by scanning electron microscopy. The minimum and fractional inhibitory concentrations of each compound were determined for planktonic cells. Next, 24-h single biofilms of C. albicans and S. mutans were treated for 24 h with the nanocompounds alone or in combination with TYR, and the antibiofilm effect was assessed through enumeration of colony forming units. Biofilm data were statistically examined using one-way ANOVA and the Kruskal–Wallis test (α = 0.05). The chemically synthesized nanocompound in combination with TYR demonstrated a synergistic effect against planktonic cells of C. albicans and S. mutans. For the nanocompound obtained through the ‘green’ route associated with TYR, a synergistic effect was observed only against C. albicans. For biofilms, only the combination obtained through the ‘green’ route + TYR demonstrated a synergistic effect against C. albicans. Our results may contribute to the development of oral care products containing AgNPs–CaGP and TYR to combat oral infections.
Department of Pediatric Dentistry and Public Health School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio, 1193
Graduate Program in Dentistry (GPD – Master’s Degree) University of Western São Paulo (UNOESTE)
Federal University of Grande Dourados
Federal University of São Carlos
University Center of Adamantina (UNIFAI)
Michigan State University
Department of Pediatric Dentistry and Public Health School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio, 1193
