Author(s):
Maquera Huacho, Patricia Milagros [UNESP] ; Rodriguez Herrero, Esteban ; Verspecht, Tim ; Pauwels, Martine ; Marcantonio, Elcio [UNESP] ; Palomari Spolidorio, Denise Madalena [UNESP] ; Teughels, Wim
Date: 2019
Persistent ID: http://hdl.handle.net/11449/190473
Origin: Oasisbr
Subject(s): carvacrol; multi-species biofilm; peri-implantitis; periodontal disease; Terpinen-4-ol; carvacrol; carvacrol; multi-species biofilm; multi-species biofilm; peri-implantitis; peri-implantitis; periodontal disease; periodontal disease; Terpinen-4-ol; Terpinen-4-ol
Description
Made available in DSpace on 2019-10-06T17:14:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01
The aim of this study was to investigate the cytotoxic activity and inhibitory effect of terpinen-4-ol (T4ol) and carvacrol against single- and multi-species biofilms. The toxicity of each compound was tested on oral keratinocytes and evaluated by XTT assay. Inhibition and eradication of single-species biofilms were analyzed by crystal violet assay and the effect on multi-species biofilm composition was evaluated by qPCR. T4ol and carvacrol did not affect the epithelial cell viability, in contrast to chlorhexidine, which showed a high cytotoxic effect. Inhibition and eradication of single-species biofilms treated with T4ol and carvacrol were observed. The same inhibitory effect was observed for multi-species biofilms, especially on periodontal pathogens. In conclusion, specific concentrations of T4ol and carvacrol without toxicity towards the epithelial cells reduced the numbers of periodontal pathogens in single- and multi-species biofilms.
Department of Oral Diagnosis and Surgery School of Dentistry São Paulo State University (Unesp)
Department of Oral Health Sciences University of Leuven & Dentistry University Hospitals Leuven
Department of Physiology and Pathology School of Dentistry São Paulo State University (Unesp)
Department of Oral Diagnosis and Surgery School of Dentistry São Paulo State University (Unesp)
Department of Physiology and Pathology School of Dentistry São Paulo State University (Unesp)
