Author(s):
Silva, Saura R. [UNESP] ; Moraes, Ana Paula ; Penha, Helen A. [UNESP] ; Julião, Maria H. M. [UNESP] ; Domingues, Douglas S. [UNESP] ; Michael, Todd P. ; Miranda, Vitor F. O. [UNESP] ; Varani, Alessandro M. [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/199855
Origin: Oasisbr
Subject(s): ABC transporters; Evolution; Genome fractionation; Transcription factors; Transposable elements; Whole-genome duplication; ABC transporters; ABC transporters; Evolution; Evolution; Genome fractionation; Genome fractionation; Transcription factors; Transcription factors; Transposable elements; Transposable elements; Whole-genome duplication; Whole-genome duplication
Description
Made available in DSpace on 2020-12-12T01:51:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01
Utricularia belongs to Lentibulariaceae, a widespread family of carnivorous plants that possess ultra-small and highly dynamic nuclear genomes. It has been shown that the Lentibulariaceae genomes have been shaped by transposable elements expansion and loss, and multiple rounds of whole-genome duplications (WGD), making the family a platform for evolutionary and comparative genomics studies. To explore the evolution of Utricularia, we estimated the chromosome number and genome size, as well as sequenced the terrestrial bladderwort Utricularia reniformis (2n = 40, 1C = 317.1-Mpb). Here, we report a high quality 304 Mb draft genome, with a scaffold NG50 of 466-Kb, a BUSCO completeness of 87.8%, and 42,582 predicted genes. Compared to the smaller and aquatic U. gibba genome (101 Mb) that has a 32% repetitive sequence, the U. reniformis genome is highly repetitive (56%). The structural differences between the two genomes are the result of distinct fractionation and rearrangements after WGD, and massive proliferation of LTR-retrotransposons. Moreover,GOenrichment analyses suggest an ongoing gene birth–death–innovation process occurring among the tandem duplicated genes, shaping the evolution of carnivory-associated functions. We also identified unique patterns of developmentally related genes that support the terrestrial life-form and body plan of U. reniformis. Collectively, our results provided additional insights into the evolution of the plastic and specialized Lentibulariaceae genomes.
Departamento de Tecnologia Faculdade de Ciências Agrárias e Veterinárias UNESP—Universidade Estadual Paulista
Centro de Ciências Naturais e Humanas Universidade Federal do ABC
Departamento de Botânica Instituto de Biociências UNESP—Universidade Estadual Paulista
J. Craig Venter Institute, La Jolla
Departamento de Biologia Aplicada � Agropecuária Faculdade de Ciências Agrárias e Veterinárias UNESP—Universidade Estadual Paulista
Departamento de Tecnologia Faculdade de Ciências Agrárias e Veterinárias UNESP—Universidade Estadual Paulista
Departamento de Botânica Instituto de Biociências UNESP—Universidade Estadual Paulista
Departamento de Biologia Aplicada � Agropecuária Faculdade de Ciências Agrárias e Veterinárias UNESP—Universidade Estadual Paulista
