Author(s):
Francisco, Karen C. A. [UNESP] ; Brandão, Pedro F. ; Ramos, Rui Miguel ; Gonçalves, Luís Moreira ; Cardoso, Arnaldo A. [UNESP] ; Rodrigues, José António
Date: 2020
Persistent ID: http://hdl.handle.net/11449/199880
Origin: Oasisbr
Subject(s): Biogenic amines; dansyl chloride; derivatisation reaction; food analysis; high-performance liquid chromatography; low molecular weight amines; salting-out assisted liquid–liquid extraction; Biogenic amines; Biogenic amines; dansyl chloride; dansyl chloride; derivatisation reaction; derivatisation reaction; food analysis; food analysis; high-performance liquid chromatography; high-performance liquid chromatography; low molecular weight amines; low molecular weight amines; salting-out assisted liquid–liquid extraction; salting-out assisted liquid–liquid extraction
Description
Made available in DSpace on 2020-12-12T01:51:49Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação para a Ciência e a Tecnologia
Biogenic amines (BAs) are compounds present in foodstuff of animal origin that not only can be harmful to the consumer but can also serve as indicators of the product's edibility status. However, BAs’ analysis is rather complicated. Derivatisation methods have been employed to make their analysis less challenging. Salting-out assisted liquid–liquid extraction (SALLE) was herein applied to decrease time that the derivatising agent dansyl chloride (DNS-Cl) requires to be effective. DNS-Cl derivates were separated and analysed by high-performance liquid chromatography with UV and fluorescence detection (HPLC-UV/FD). It was by this way possible to analyse methylamine, dimethylamine, ethylamine, β-phenylethylamine, isopentylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine in a straightforward manner in several fish-based and meat-based samples, obtaining results in the order of magnitude of about a few mg per kg. All calibrations curves had r2 higher than 0.99, and limits of detection ranged from 0.0075 to 1.6 mg L−1.
Departamento de Química Analítica Instituto de Química Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Av. Prof. Francisco Degni, 55
REQUIMTE/LAQV Departamento de Química e Bioquímica Faculdade de Ciências da Universidade do Porto (FCUP), Rua do Campo Alegre, 687
Departamento de Química Fundamental Instituto de Química Universidade de São Paulo (USP), Avenida Prof. Lineu Prestes, 748
Departamento de Química Analítica Instituto de Química Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Av. Prof. Francisco Degni, 55
FAPESP: 2018/14425-7
CAPES: 88887.138029/2017
Fundação para a Ciência e a Tecnologia: NORTE-01-0145-FEDER-00011
Fundação para a Ciência e a Tecnologia: PD/BD/142931/2018
Fundação para a Ciência e a Tecnologia: POCI/01/0145/FEDER/007265
Fundação para a Ciência e a Tecnologia: UID/QUI/50006/2013
