Autor(es):
Gonçalves, Luiz Ricardo [UNESP] ; Herrera, Heitor Miraglia ; Nantes, Wesley Arruda Gimenes ; Santos, Filipe Martins ; Porfírio, Grasiela Edith de Oliveira ; Barreto, Wanessa Teixeira Gomes ; de Macedo, Gabriel Carvalho ; Assis, Willian de Oliveira ; Campos, João Bosco Vilela ; da Silva, Thiago Merighi Vieira [UNESP] ; Mariano, Leticia Colovatti [UNESP] ; Barros-Battesti, Darci Moraes [UNESP] ; Machado, Rosangela Zacarias [UNESP] ; André, Marcos Rogério [UNESP]
Data: 2020
Identificador Persistente: http://hdl.handle.net/11449/199891
Origem: Oasisbr
Assunto(s): Capybaras; Hemotropic mycoplasmas; Lice; Opossum; Phylogenetic analysis; Rattus rattus and Ticks; Capybaras; Capybaras; Hemotropic mycoplasmas; Hemotropic mycoplasmas; Lice; Lice; Opossum; Opossum; Phylogenetic analysis; Phylogenetic analysis; Rattus rattus and Ticks; Rattus rattus and Ticks
Descrição
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul
Globally, hemotropic mycoplasmas (hemoplasmas) comprise an emerging or remerging bacteria group that attaches to red blood cells of several mammal's species and in some cases, causing hemolytic anemia. Herein, we assessed the occurrence, genetic diversity, the factors coupled to mammals infection, and the phylogeographic distribution of hemoplasmas in sylvatic and synanthropic mammals and their associated ectoparasites from Brazil. We collected spleen and/or blood samples from synanthropic rodents (Rattus rattus [N = 39] and Mus musculus [N = 9]), sylvatic rodents (Hydrochoerus hydrochaeris [N = 14]) and opossums (Didelphis albiventris [N = 43]). In addition, ticks (Amblyomma spp. [N = 270] and lice (Polyplax spinulosa [N = 6]) specimens were also sampled. Using a PCR targeting the 16S rRNA region, out of 48 small rodents, 14 capybaras and 43 opossums DNA samples, hemoplasma DNA was found in 25%, 50%, and 32.5% animals, respectively. Besides, we reported hemoplasma DNA in Amblyomma sp. (22.2% [2/9]) and lice (100% [2/2]) pools samples from rats, and one female A. sculptum DNA sample (3% [1/33]) obtained from a capybara. Additionally, and in agreement with ML analysis, the network analyses showed a clear phylogenetic separation among the hemoplasmas genotypes found in the different host species sampled, thus, suggesting the absence of cross-species hemoplasmas transmission between the mammals trapped. Finally, using the NTC network analysis, we reported the same 16S rRNA Mycoplasma genotype circulating in Rattus sampled in Brazil, Hungary, and Japan.
Agricultural Microbiology Program School of Agrarian and Veterinary Sciences (FCAV/UNESP), Jaboticabal
Department of Veterinary Pathology School of Agrarian and Veterinary Sciences (FCAV/UNESP), Jaboticabal
Dom Bosco Catholic University (UCDB)
Graduate Program of Natural Resources Federal University of Mato Grosso do Sul
Agricultural Microbiology Program School of Agrarian and Veterinary Sciences (FCAV/UNESP), Jaboticabal
Department of Veterinary Pathology School of Agrarian and Veterinary Sciences (FCAV/UNESP), Jaboticabal
FAPESP: 2018/02753-0
Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul: 59/300.187/2016
