Author(s): Tanamati, Fernanda [UNESP] ; Taylor, Jeremy F. ; Behura, Susanta K. ; Santos, Daniel J.A. ; Stafuzza, Nedenia B. ; Andrade, Willian B.F. [UNESP] ; Gasparino, Eliane ; Tonhati, Humberto [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/199963
Origin: Oasisbr
Subject(s): Bubalus bubalis; cathelicidin-3; milk whey protein; proteomics; Bubalus bubalis; Bubalus bubalis; cathelicidin-3; cathelicidin-3; milk whey protein; milk whey protein; proteomics; proteomics
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The aim of this study was to characterize the proteins present in milk whey from buffaloes with and without subclinical mastitis using a proteomic approach to identify differentially expressed proteins as potential biomarkers for this disease. Whey from Murrah buffaloes with subclinical mastitis was compared with whey from healthy animals using liquid chromatography–tandem mass spectrometry. The annotated protein databases for Bubalus bubalis and Bos taurus were used in the analysis, and the gene annotations from the buffalo and bovine reference assemblies were also used. After integrating gene annotations from both buffaloes and bovines, a total of 1,033 proteins were identified, of which 156 were differentially expressed. Eighteen biological processes were annotated with Gene Ontology. Cathelicidin-3 was identified as a potential biomarker for subclinical mastitis. These results are important to the characterization of mastitis in the buffalo mammary gland and may aid in the development of tools for early diagnosis.
Department of Animal Science São Paulo State University (FCAV/UNESP)
Division of Animal Sciences University of Missouri
Department of Animal and Avian Sciences University of Maryland
Institute of Animal Science (IZ)
Department of Animal Sciences Maringá State University (UEM)
Department of Animal Science São Paulo State University (FCAV/UNESP)
