Author(s):
Dini, Caroline ; Nagay, Bruna E. ; Cordeiro, Jairo M. ; da Cruz, Nilson C. [UNESP] ; Rangel, Elidiane C. [UNESP] ; Ricomini-Filho, Antônio P. ; de Avila, Erica D. [UNESP] ; Barão, Valentim A.R.
Date: 2020
Persistent ID: http://hdl.handle.net/11449/199970
Origin: Oasisbr
Subject(s): Biofilm; Dental implants; Photofunctionalization; Plasma electrolytic oxidation; Titanium; Ultraviolet rays; Biofilm; Biofilm; Dental implants; Dental implants; Photofunctionalization; Photofunctionalization; Plasma electrolytic oxidation; Plasma electrolytic oxidation; Titanium; Titanium; Ultraviolet rays; Ultraviolet rays
Description
Made available in DSpace on 2020-12-12T01:54:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-05-01
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Photofunctionalization mediated by ultraviolet (UV) rays changes the physico-chemical characteristics of titanium (Ti) and improves the biological activity of dental implants. However, the role of UV-mediated photofunctionalization of biofunctional Ti surfaces on the antimicrobial and photocatalytic activity remains unknown and was investigated in this study. Commercially pure titanium (cpTi) discs were divided into four groups: (1) machined samples without UV light application [cpTi UV−]; (2) plasma electrolytic oxidation (PEO) treated samples without UV light application [PEO UV−]; (3) machined samples with UV light application [cpTi UV+]; and (4) PEO-treated samples with UV light application [PEO UV+]. The surfaces were characterized according to their morphology, roughness, crystalline phase, chemical composition and wettability. The photocatalytic activity and proteins adsorption were measured. For the microbiological assay, Streptococcus sanguinis was grown on the disc surfaces for 1 h and 6 h, and the colony forming units and bacterial organization were evaluated. In addition, to confirm the non-cytotoxic effect of PEO UV +, human gingival fibroblast (HGF) cells were cultured in a monolayer onto each material surface and the cells viability and proliferation evaluated by a fluorescent cell staining method. PEO treatment increased the Ti surface roughness and wettability (p < 0.05). Photofunctionalization reduced the hydrocarbon concentration and enhanced human blood plasma proteins and albumin adsorption mainly for the PEO-treated surface (p < 0.05). PEO UV+ also maintained higher wettability values for a longer period and provided microbial reduction at 1 h of bacterial adhesion (p = 0.012 vs. PEO UV-). Photofunctionalization did not increase the photocatalytic activity of Ti (p > 0.05). Confocal microscopy analyses demonstrated that PEO UV+ had no cell damage effect on HGF cells growth even after 24 h of incubation. The photofunctionalization of a biofunctional PEO coating seems to be a promising alternative for dental implants as it increases blood plasma proteins adsorption, reduces initial bacterial adhesion and presents no cytotoxicity effect.
Department of Prosthodontics and Periodontology Piracicaba Dental School University of Campinas (UNICAMP), Av. Limeira, 901
Institute of Science and Technology São Paulo State University (UNESP), Av. Três de Março, 511
Department of Physiological Science Piracicaba Dental School University of Campinas (UNICAMP), Av. Limeira, 901
Department of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University (UNESP), R. Humaitá, 1680
Institute of Science and Technology São Paulo State University (UNESP), Av. Três de Março, 511
Department of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University (UNESP), R. Humaitá, 1680
CAPES: 001
FAPESP: 2016/11470-6
FAPESP: 2017/01320-0
