Author(s):
Alpire, Maria Esther Suarez ; de Camargo, Elaine Aparecida [UNESP] ; Cardoso, Caroline Margonato ; Salvadori, Daisy Maria Favero [UNESP] ; Pereira, Camilo Dias Seabra ; Ribeiro, Daniel Araki
Date: 2020
Persistent ID: http://hdl.handle.net/11449/199989
Origin: Oasisbr
Subject(s): Comet assay; Cytotoxicity; Genotoxicity; Micronucleus assay; Santos-São Vicente estuary; Comet assay; Comet assay; Cytotoxicity; Cytotoxicity; Genotoxicity; Genotoxicity; Micronucleus assay; Micronucleus assay; Santos-São Vicente estuary; Santos-São Vicente estuary
Description
Made available in DSpace on 2020-12-12T01:54:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-04-01
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
The aim of the study was to evaluate cyto- and genotoxic effects in populations living in subnormal clusters in Santos São Vicente estuary. For in vivo study, samples of buccal mucosa and peripheral blood cells were collected. Micronucleus assay and single-cell gel (comet) assay were performed. For in vitro study, Chinese ovary hamster (CHO) cells were exposed to contaminated water. The results showed that people living in the contaminated estuary have increased DNA damage in oral mucosa and peripheral blood cells, as detected in the micronucleus and comet assays respectively. In addition, estuarine water was able to promote cytotoxicity at the highest concentrations, as well as decrease the number of cells in the G1 phase. In summary, our results indicate that water from the Santos-São Vicente estuary is capable of inducing cytogenotoxicity in mammalian cells in vivo and in vitro.
Departamento de Biociências Universidade Federal de São Paulo UNIFESP, Av. Ana Costa, 95, Vila Mathias
Department of Pathology Botucatu Medical School São Paulo State University UNESP
Department of Sea Sciences Federal University of São Paulo UNIFESP Campus Baixada Santista
Department of Pathology Botucatu Medical School São Paulo State University UNESP
CNPq: 001
