Author(s):
Dos Santos Martins, Evelin [UNESP] ; Oliveira, Jonata Augusto [UNESP] ; Franchin, Taísa Busaranho [UNESP] ; Silva, Bruna Cristina Ulian [UNESP] ; Cândido, Caroline Damico [UNESP] ; Peccinini, Rosângela Gonçalves [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/200000
Origin: Oasisbr
Subject(s): AIDS; Bioanalytical method; Efavirenz; Pharmacokinetics; UHPLC; AIDS; AIDS; Bioanalytical method; Bioanalytical method; Efavirenz; Efavirenz; Pharmacokinetics; Pharmacokinetics; UHPLC; UHPLC
Description
Made available in DSpace on 2020-12-12T01:54:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-10-10
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
A simple and rapid ultra-high-performance liquid chromatography (UHPLC) method for determination of efavirenz (EFV) in plasma was developed and applied in a preclinical pharmacokinetic study. The method involves only addition of acetonitrile to precipitation of plasma proteins followed by solvent evaporation. The mobile phase consisted of methanol, acetonitrile and 0.1 M formic acid (20:50:30) at a flow rate of 0.3 mL/min with run time of 5 min. A CSH C18 column and a UHPLC-UV system operating at 245 nm were used. There was a linear response in the range of 0.078 to 10 μg/mL, and the equation was obtained by weighting (1/x2) with r2 = 0.9965. The pharmacokinetic disposition of EFV was investigated in rabbits (two groups, n = 7) following a single intravenous administration (IV group) at a dose of 2.7 mg/kg and a single oral administration (oral group) of EFV co-administered with lamivudine (3TC) and tenofovir (TNF) at a dose of 50, 25 and 25 mg, respectively. The study demonstrated the applicability of the method for determination of EFV in plasma without interference from other co-administered drugs, and the pharmacokinetic parameters were calculated. The method showed advantages over other methods in the literature, such as simplicity of sample processing and fast results.
Department of Natural Active Principles and Toxicology School of Pharmaceutical Sciences Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP
Department of Natural Active Principles and Toxicology School of Pharmaceutical Sciences Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP
