Author(s):
Mariani, Noemia A.P. [UNESP] ; Camara, Aline C. [UNESP] ; Silva, Alan Andrew S. [UNESP] ; Raimundo, Tamiris R.F. [UNESP] ; Andrade, Juliana J. [UNESP] ; Andrade, Alexandre D. [UNESP] ; Rossini, Bruno C. [UNESP] ; Marino, Celso L. [UNESP] ; Kushima, Hélio [UNESP] ; Santos, Lucilene D. [UNESP] ; Silva, Erick J.R. [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/200050
Origin: Oasisbr
Subject(s): EPPIN; Male contraception; Mouse; Spermatozoa; SVS2; EPPIN; EPPIN; Male contraception; Male contraception; Mouse; Mouse; Spermatozoa; Spermatozoa; SVS2; SVS2
Description
Made available in DSpace on 2020-12-12T01:56:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-04-15
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
EPPIN is a sperm-surface drug target for male contraception. Here we investigated EPPIN-interacting proteins in mouse spermatozoa. We showed that EPPIN is an androgen-dependent gene, expressed in the testis and epididymis, but also present in the vas deferens, seminal vesicle and adrenal gland. Mature spermatozoa presented EPPIN staining on the head and flagellum. Immunoprecipitation of EPPIN from spermatozoa pre-incubated with seminal vesicle fluid (SVF) followed by LC-MS/MS or Western blot revealed the co-immunoprecipitation of SVS2, SVS3A, SVS5 and SVS6. In silico and Far-Western blot approaches demonstrated that EPPIN binds SVS2 in a protein network with other SVS proteins. Immunofluorescence using spermatozoa pre-incubated with SVF or recombinant SVS2 demonstrated the co-localization of EPPIN and SVS2 both on sperm head and flagellum. Our data show that EPPIN's roles in sperm function are conserved between mouse and human, demonstrating that the mouse is a suitable experimental model for translational studies on EPPIN.
Department of Pharmacology Institute of Biosciences São Paulo State University (UNESP), Botucatu-SP
Center for the Study of Venoms of Venomous Animals (CEVAP) São Paulo State University (UNESP), Botucatu-SP
Biotechnology Institute (IBTEC) São Paulo State University (UNESP), Botucatu-SP
Department of Genetics Institute of Biosciences São Paulo State University (UNESP), Botucatu-SP
Graduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP), Botucatu-SP
Department of Pharmacology Institute of Biosciences São Paulo State University (UNESP), Botucatu-SP
Center for the Study of Venoms of Venomous Animals (CEVAP) São Paulo State University (UNESP), Botucatu-SP
Biotechnology Institute (IBTEC) São Paulo State University (UNESP), Botucatu-SP
Department of Genetics Institute of Biosciences São Paulo State University (UNESP), Botucatu-SP
Graduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP), Botucatu-SP
FAPESP: #2015/08227-0
FAPESP: #2016/23025-7
FAPESP: #2017/11363-8
CAPES: 001
