Autor(es):
Soares, Diana Gabriela ; Bordini, Ester Alves Ferreira [UNESP] ; Cassiano, Fernanda Balestrero [UNESP] ; Bronze-Uhle, Erika Soares ; Pacheco, Leandro Edgar ; Zabeo, Giovana ; Hebling, Josimeri [UNESP] ; Lisboa-Filho, Paulo Noronha [UNESP] ; Bottino, Marco Cicero ; de Souza Costa, Carlos Alberto [UNESP]
Data: 2020
Identificador Persistente: http://hdl.handle.net/11449/200070
Origem: Oasisbr
Assunto(s): calcium hydroxide; chitosan; dental pulp; dentin; porous scaffolds; calcium hydroxide; calcium hydroxide; chitosan; chitosan; dental pulp; dental pulp; dentin; dentin; porous scaffolds; porous scaffolds
Descrição
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
The aim of this study was to develop a highly porous calcium-containing chitosan scaffold suitable for dentin regeneration. A calcium hydroxide (Ca[OH]2) suspension was used to modulate the degree of porosity and chemical composition of chitosan scaffolds. The chitosan solution concentration and freezing protocol were adjusted to optimize the porous architecture using the phase-separation technique. Scanning electron microscopy/energy-dispersive spectroscopy demonstrated the fabrication of a highly porous calcium-linked chitosan scaffold (CH-Ca), with a well-organized and interconnected porous network. Scaffolds were cross-linked on glutaraldehyde (GA) vapor. Following a 28-day incubation in water, cross-linked CH scaffold had no changes on humid mass, and CH-Ca featured a controlled degradability profile since the significant humid mass loss was observed only after 21 (26.0%) and 28 days (42.2%). Fourier-transform infrared spectroscopy indicated the establishment of Schiff base on cross-linked scaffolds, along with calcium complexation for CH-Ca. Cross-linked CH-Ca scaffold featured a sustained Ca2+ release up to 21 days in a humid environment. This porous and stable architecture allowed for human dental pulp cells (HDPCs) to spread throughout the scaffold, with cells exhibiting a widely stretched cytoplasm; whereas, the cells seeded onto CH scaffold were organized in clusters. HDPCs seeded onto CH-Ca featured significantly higher ALP activity, and gene expressions for ALP, Col1, DMP-1, and DSPP in comparison to CH, leading to a significant 3.5 times increase in calcium-rich matrix deposition. In sum, our findings suggest that CH-Ca scaffolds are attractive candidates for creating a highly porous and bioactive substrate for dentin tissue engineering.
Department of Operative Dentistry Endodontics and Dental Materials Bauru School of Dentistry Sao Paulo University - USP
Department of Physiology and Pathology Araraquara School of Dentistry Univ. Estadual Paulista - UNESP
Department of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry Univ. Estadual Paulista – UNESP
Department of Physics School of Sciences Univ. Estadual Paulista – UNESP
Department of Cariology Restorative Sciences Endodontics School of Dentistry University of Michigan
Department of Physiology and Pathology Araraquara School of Dentistry Univ. Estadual Paulista - UNESP
Department of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry Univ. Estadual Paulista – UNESP
Department of Physics School of Sciences Univ. Estadual Paulista – UNESP
CAPES: 001
FAPESP: 2016/15674-5
