Author(s):
Lima, Bruna Jacomel Favoreto de Souza ; Voidaleski, Morgana Ferreira ; Gomes, Renata Rodrigues ; Fornari, Gheniffer ; Soares, Jade Mariane Barbosa ; Bombassaro, Amanda ; Schneider, Gabriela Xavier ; Soley, Bruna da Silva ; de Azevedo, Conceição de Maria Pedrozo e Silva ; Menezes, Cristiano ; Moreno, Leandro Ferreira ; Attili-Angelis, Derlene [UNESP] ; Klisiowicz, Débora do Rocio ; de Hoog, Sybren ; Vicente, Vânia Aparecida
Date: 2020
Persistent ID: http://hdl.handle.net/11449/200102
Origin: Oasisbr
Subject(s): Atta laevigata; Black yeasts; Fonsecaea pedrosoi; Melipona flavolineata; Nasutitermes sp.; Scaptotrigona postica; Atta laevigata; Atta laevigata; Black yeasts; Black yeasts; Fonsecaea pedrosoi; Fonsecaea pedrosoi; Melipona flavolineata; Melipona flavolineata; Nasutitermes sp.; Nasutitermes sp.; Scaptotrigona postica; Scaptotrigona postica
Description
Made available in DSpace on 2020-12-12T01:57:45Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-03-01
National Council for Scientific Research
Federal Agency for Scientific Organizations
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Chromoblastomycosis is a neglected disease characterized by cutaneous, subcutaneous or disseminated lesions. It is considered an occupational infectious disease that affects mostly rural workers exposed to contaminated soil and vegetal matter. Lesions mostly arise after a traumatic inoculation of herpotrichiellaceous fungi from the Chaetothyriales order. However, the environmental niche of the agents of the disease remains obscure. Its association with insects has been predicted in a few studies. Therefore, the present work aimed to analyze if social insects, specifically ants, bees, and termites, provide a suitable habitat for the fungi concerned. The mineral oil flotation method was used to isolate the microorganisms. Nine isolates were recovered and phylogenetic analysis identified two strains as potential agents of chromoblastomycosis, i.e., Fonsecaea pedrosoi CMRP 3076, obtained from a termite nest (n = 1) and Rhinocladiella similis CMRP 3079 from an ant exoskeleton (n = 1). In addition, we also identified Fonsecaea brasiliensis CMRP 3445 from termites (n = 1), Exophiala xenobiotica CMRP 3077 from ant exoskeleton (n = 1), Cyphellophoraceae CMRP 3103 from bees (n = 1), Cladosporium sp. CMRP 3119 from bees (n = 1), Hawksworthiomyces sp. CMRP 3102 from termites (n = 1), and Cryptendoxyla sp. from termites (n = 2). The environmental isolate of F. pedrosoi CMRP 3076 was tested in two animal models, Tenebrio molitor and Wistar rat, for its pathogenic potential with fungal retention in T. molitor tissue. In the Wistar rat, the cells resembling muriform cells were observed 30 d after inoculation.
Graduate Program in Microbiology Parasitology and Pathology Federal University of Paraná
University Center Campo Real
Federal University of Paraná
Graduate Program in Pharmacology UFPR
Medical Department Federal University of Maranhão
Embrapa Amazônia Oriental CPATU
Westerdijk Fungal Biodiversity Institute
Department of Biochemistry and Microbiology Institute of Biological Sciences UNESP-São Paulo State University
Division of Microbial Resources CPQBA University of Campinas
Center of Expertise in Mycology of Radboud University Medical Center Canisius Wilhelmina Hospital
Department of Biochemistry and Microbiology Institute of Biological Sciences UNESP-São Paulo State University
CNPq: 312811/2018–7
CAPES: Brazil
CAPES: Finance Code 001, Brasilia
