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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Keratin is the main protein in hair strands. The process of dyeing hair with permanent dyes is quite complex and involves oxidative reactions between precursors, such as p-toluenediamine (PTD) and p-aminophenol (PAP), and coupler agents, in alkaline and oxidative medium, inside the hair. The electrochemical behaviour of native and denatured human hair keratin, assessed by using a keratin multilayer film adsorbed on glassy carbon electrode, and its interaction with hair dye precursors, PTD and PAP, by using electrochemical techniques, was investigated. Native and denatured keratin electrochemical oxidation showed two oxidation peaks; the first was first attributed to the cysteine amino acid residues, and the second to the cysteine and methionine amino acids residues. The PTD- and PAP-keratin-hair dye interactions induced damage, causing the unfolding of the keratin morphological structure, and new additional peaks of the cysteine and tyrosine amino acid residues were revealed.

Department of Chemistry Faculty of Science and Technology University of Coimbra, Rua Larga

Department of Analytical Chemistry Institute of Chemistry National Institute of Alternative Technologies for Detection Toxicological Assessment and Removal of Micropollutants and Radioactive (INCT-DATREM) São Paulo State University (UNESP), Av. Prof. Francisco Degni, 55

Department of Analytical Chemistry Institute of Chemistry National Institute of Alternative Technologies for Detection Toxicological Assessment and Removal of Micropollutants and Radioactive (INCT-DATREM) São Paulo State University (UNESP), Av. Prof. Francisco Degni, 55

FAPESP: 2015/12998-1

FAPESP: 2018/12449-6

FAPESP: 232296/2014-6