Autor(es):
Da Silva, Marcelo J. [UNESP] ; Fogarin Destro, Raquel [UNESP] ; Gazoni, Thiago [UNESP] ; Narimatsu, Hideki [UNESP] ; Pereira Dos Santos, Paulo S. [UNESP] ; Haddad, Célio F.B. [UNESP] ; Parise-Maltempi, Patricia P. [UNESP]
Data: 2020
Identificador Persistente: http://hdl.handle.net/11449/200210
Origem: Oasisbr
Assunto(s): Amphibia; Cytogenetics; Fluorescence in situ hybridization; Proceratophrys boiei; RepeatExplorer; Amphibia; Amphibia; Cytogenetics; Cytogenetics; Fluorescence in situ hybridization; Fluorescence in situ hybridization; Proceratophrys boiei; Proceratophrys boiei; RepeatExplorer; RepeatExplorer
Descrição
Made available in DSpace on 2020-12-12T02:00:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-04-01
Most eukaryotic genomes contain substantial portions of repetitive DNA sequences. These are located primarily in highly compacted heterochromatin and, in many cases, are one of the most abundant components of the sex chromosomes. In this sense, the anuran Proceratophrys boiei represents an interesting model for analyses on repetitive sequences by means of cytogenetic techniques, since it has a karyotype with large blocks of heterochromatin and a ZZ/ZW sex chromosome system. The present study describes, for the first time, families of satellite DNA (satDNA) in the frog P. boiei. Its genome size was estimated at 1.6 Gb, of which 41% correspond to repetitive sequences, including satDNAs, rDNAs, transposable elements, and other elements characterized as non-repetitive. The satDNAs were mapped by FISH in the centromeric and pericentromeric regions of all chromosomes, suggesting a possible involvement of these sequences in centromere function. SatDNAs are also present in the W sex chromosome, occupying the entire heterochromatic area, indicating a probable contribution of this class of repetitive DNA to the differentiation of the sex chromosomes in this species. This study is a valuable contribution to the existing knowledge on repetitive sequences in amphibians. We show the presence of repetitive DNAs, especially satDNAs, in the genome of P. boiei that might be of relevance in genome organization and regulation, setting the stage for a deeper functional genome analysis of Proceratophrys.
Departamento de Biologia Instituto de Biociências Universidade Estadual Paulista - UNESP, Av. 24A, 1515
Departamento de Zoologia e Centro de Aquicultura Instituto de Biociências Universidade Estadual Paulista - UNESP
Departamento de Biologia Instituto de Biociências Universidade Estadual Paulista - UNESP, Av. 24A, 1515
Departamento de Zoologia e Centro de Aquicultura Instituto de Biociências Universidade Estadual Paulista - UNESP
