Author(s):
Vesentini, Giovana [UNESP] ; Barbosa, Angélica M.P. [UNESP] ; Damasceno, Débora C. [UNESP] ; Marini, Gabriela [UNESP] ; Piculo, Fernanda [UNESP] ; Matheus, Selma M.M. [UNESP] ; Hallur, Raghavendra L.S. [UNESP] ; Nunes, Sthefanie K. [UNESP] ; Catinelli, Bruna B. [UNESP] ; Magalhães, Claudia G. [UNESP] ; Costa, Roberto [UNESP] ; Abbade, Joelcio F. [UNESP] ; Corrente, José E. [UNESP] ; Calderon, Iracema M.P. [UNESP] ; Rudge, Marilza V.C. [UNESP] ; Souza, F. P. [UNESP] ; Hijas, A. ; Sobrevia, L. ; Palma, M. S. [UNESP] ; Graeff, C. F.O. [UNESP] ; Arni, R. K. [UNESP] ; Herculano, R. D. [UNESP] ; Salvadori, D. F. [UNESP] ; Dal Pai Silva, M. [UNESP] ; Lima, S. A.M. [UNESP] ; Emanueli, C. ; Felisbino, S. [UNESP] ; Barbosa, W. [UNESP] ; Atallah, A. ; Girão, M. J.B. ; Di Bella, Z. ; Uchoa, S. M. ; Payão, S. ; Berghman, B. ; De Bie, R. ; Junginger, B. ; Alves, F. C.B. [UNESP] ; Rossignoli, P. S. [UNESP] ; Prudencio, C. B. [UNESP] ; Orlandi, M. I.G. [UNESP] ; Gonçalves, M. I. [UNESP] ; Sarmento, B. V. [UNESP] ; Pinheiro, F. A. [UNESP] ; Sartorão, C. I. [UNESP] ; Quiroz, S. B.C.V. [UNESP] ; Reyes, D. R.A. [UNESP] ; Enriquez, E. M.A. [UNESP] ; Oliveira, R. G. [UNESP] ; Floriano, J. F. [UNESP] ; Marcondes, J. P.C. [UNESP] ; Costa, S. M.B. [UNESP] ; Dangió, T. D. [UNESP] ; Pascon, T. [UNESP] ; Melo, J. V.F. [UNESP] ; Takano, L. [UNESP] ; Reis, F. V.D.S. [UNESP] ; Caldeirão, T. D. [UNESP] ; Carr, A. M. [UNESP] ; Garcia, G. A. [UNESP] ; Rabadan, G. B. [UNESP] ; Bassin, H. C.M. ; Suyama, K. S. [UNESP] ; Damasceno, L. N. [UNESP] ; Takemoto, M. L.S. [UNESP] ; Menezes, M. D. [UNESP] ; Bussaneli, D. G. [UNESP] ; Nogueira, V. K.C. [UNESP] ; Lima, P. R. [UNESP] ; Lourenço, I. O. [UNESP] ; Marostica De Sá, J. [UNESP] ; Megid, R. A. [UNESP] ; Caruso, I. P. [UNESP] ; Rasmussen, L. T. ; Prata, G. M. ; Arantes, M. A. [UNESP] ; Ferraz, G. A.R. [UNESP] ; Camargo, L. P. [UNESP] ; Kron, M. R. [UNESP] ; Nunes, H. R.C. [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/200259
Origin: Oasisbr
Description
Made available in DSpace on 2020-12-12T02:01:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-04-01
Associação Brasileira de Saúde Coletiva
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Background and objective In the present study, we compared the effect of diabetic pregnancy on the rectus abdominis muscle (RAM) in humans and rats. We hypothesized that our animal model could provide valuable information about alterations in the RAM of women with Gestational Diabetes (GDM). Method Newborns female rats (n = 10/group) were administered streptozotocin (100 mg/kg body weight) subcutaneously and were mated on reaching adulthood, to develop the mild hyperglycemic pregnant (MHP) rat model. At the end of pregnancy, the mothers were sacrificed, and the RAM tissue was collected. Pregnant women without GDM (non-GDM group; n = 10) and those diagnosed with GDM (GDM group; n = 8) and undergoing treatment were recruited, and RAM samples were obtained at C-section. The RAM architecture and the distribution of the fast and slow fibers and collagen were studied by immunohistochemistry. Results No statistically significant differences in the maternal and fetal characters were observed between the groups in both rats and women. However, significant changes in RAM architecture were observed. Diabetes in pregnancy increased the abundance of slow fibers and decreased fast fiber number and area in both rats and women. A decrease in collagen distribution was observed in GDM women; however, a similar change was not observed in the MHP rats. Conclusion Our results indicated that pregnancy- associated diabetes- induced similar structural adaptations in the RAM of women and rats with slight alterations in fiber type number and area. These findings suggest that the MHP rat model can be used for studying the effects of pregnancy-associated diabetes on the fiber structure of RAM.
Perinatal Diabetes Research Center University Hospital Botucatu Medical School Univ. Estadual Paulista-UNESP
Department of Gynecology and Obstetrics São Paulo State University (UNESP) Botucatu Medical School
Department of Physiotherapy and Occupational Therapy São Paulo State University (UNESP) School of Philosophy and Sciences
Department of Health Sciences Universidade Sagrado Coração
Department of Anatomy São Paulo State University (UNESP) Institute of Biosciences
Department of Biostatistics São Paulo State University (UNESP) Bioscience Institute
Universidade Estadual Paulista
Case Western Reserve University
Pontificia Universidad Catolica de Chile
Imperial College London
Universidade Federal de São Paulo
Universidade Católica de Pernambuco
Faculdade de Medicina de Marília
Maastrich University
Charité Universitätsmedizin Berlin
Universidade do Sagrado Coração
Faculdades Integradas de Bauru
Perinatal Diabetes Research Center University Hospital Botucatu Medical School Univ. Estadual Paulista-UNESP
Department of Gynecology and Obstetrics São Paulo State University (UNESP) Botucatu Medical School
Department of Physiotherapy and Occupational Therapy São Paulo State University (UNESP) School of Philosophy and Sciences
Department of Anatomy São Paulo State University (UNESP) Institute of Biosciences
Department of Biostatistics São Paulo State University (UNESP) Bioscience Institute
Universidade Estadual Paulista
FAPESP: #2014/26852-6
FAPESP: #2016/01743-5
FAPESP: #2016/09710-9
