Author(s):
Freitas, Juliane Silberschmidt ; Pereira, Thiago Scremin Boscolo ; Boscolo, Camila Nomura Pereira ; Garcia, Mariana Navarro [UNESP] ; de Oliveira Ribeiro, Ciro Alberto ; de Almeida, Eduardo Alves
Date: 2020
Persistent ID: http://hdl.handle.net/11449/200369
Origin: Oasisbr
Subject(s): Antioxidant enzymes; Biotransformation; Fish; Histopathology; Lubricating oil; Antioxidant enzymes; Antioxidant enzymes; Biotransformation; Biotransformation; Fish; Fish; Histopathology; Histopathology; Lubricating oil; Lubricating oil
Description
Made available in DSpace on 2020-12-12T02:04:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-08-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Lubricant oils are among oil-based products that are not fully consumed during its use, thereby producing non-biodegradable residues which can cause contamination of natural systems. This study evaluated the toxicity of new and used lubricating oil (0.01 and 0.1 mL L−1) in adult Nile tilapia (Oreochromis niloticus), by assessing the effects on oxidative stress, biotransformation enzymes (liver and gills), and histopathological alterations on hepatic and pancreatic tissues after 3 and 7 days of exposure. Results showed that 3-days exposure to 0.1 mL L−1 of used and new lubricating oil increased the activity of superoxide dismutase (SOD) and malondialdehyde (MDA) levels in liver of O. niloticus, respectively. In gills, catalase (CAT) was decreased in fish exposed to 0.1 mL L−1 of non-used oil after 3 days, but pronounced increases in CAT was detected after 7 days-exposure to both new and used oil. Shorter exposure to both concentrations of new and used oil also raised glutathione-S-transferase activity (GST) in gills. Ethoxyresorufin-O-deethylase (EROD) was induced in liver of fish exposed to 0.1 mL L−1of used oil after 3 and 7 days, however a reduced response of this enzyme was detected in gills of animals from both oil treatments. In vitro analysis showed that hepatic EROD was inhibited by lubricating oil exposures, with more pronounced responses in treatments containing used oil. Hepatic lesions, such as cytoplasmic vacuolization, nuclei abnormally, changes in hepatocytes shape, steatosis, cholestasis, eosinophilic inclusions and necrosis were mainly increased by 7 days exposure to used lubricating oil at higher concentration.
Department of Biology Minas Gerais State University (UEMG), R. Ver. Geraldo Moisés da Silva, s/n - Universitário
UNIRP - University Center of Rio Preto
FACERES - Morfofunctional Laboratory FACERES Medical School
Department of Chemistry and Environmental Science Universidade Estadual Paulista (IBILCE/UNESP), R. Critóvão Colombo, 2265
Department of Cell Biology Universidade Federal do Paraná, Centro Politécnico, 19031
Department of Natural Sciences Fundação Universidade Regional de Blumenau, Av. Antonio da Veiga 140, Itoupava Seca
Department of Chemistry and Environmental Science Universidade Estadual Paulista (IBILCE/UNESP), R. Critóvão Colombo, 2265
FAPESP: 2006/03873-1
