Author(s): Sarmiento-Machado, Luis Manuel [UNESP] ; Romualdo, Guilherme Ribeiro [UNESP] ; Zapaterini, Joyce Regina [UNESP] ; Tablas, Mariana Baptista [UNESP] ; Fernandes, Ana Angélica Henrique [UNESP] ; Moreno, Fernando Salvador ; Barbisan, Luís Fernando [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/200428
Origin: Oasisbr
Made available in DSpace on 2020-12-12T02:06:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01
Capsaicin (CPS), an ingredient of Capsicum plants, has anti-inflammatory, antioxidant and antitumoral properties. The mechanisms of CPS on hepatocarcinogenesis preclinical bioassays are not described. Thus, the protective effects CPS were evaluated in the early stages of chemically-induced hepatocarcinogenesis. Male Wistar rats received diet containing 0.01% or 0.02% CPS for 3 weeks. Afterwards, animals received a dose of hepatocarcinogen diethylnitrosamine (DEN, 100 mg/kg body weight). From weeks 4–12, groups had their diet replaced by a 0.05% phenobarbital supplemented one to promote DEN-induced preneoplastic lesions. Animals were euthanized 24 h after DEN administration (n = 5/group) or at week 12 (n = 9/group). The estimated CPS intake in rats resembled human consumption. At the end of week 3, dietary 0.02% CPS attenuated DEN-induced oxidative damage and, consequently, hepatocyte necrosis by reducing serum alanine aminotransferase levels, liver CD68-positive macrophages, lipid peroxidation, while increasing antioxidant glutathione system. Additionally, 0.02% CPS upregulated vanilloid Trpv1 receptor and anti-inflammatory epoxygenase Cyp2j4 genes in the liver. Ultimately, previous 0.02% CPS intake decreased the number of GST-P-positive preneoplastic lesions at week 12. Thus, CPS attenuated preneoplastic lesion development, primarily by diminishing DEN-induced oxidative liver injury. Findings indicate that CPS is a promising chemopreventive agent when administered after and during the early stages of hepatocarcinogenesis.
Department of Morphology Biosciences Institute São Paulo State University (UNESP)
Department Pathology Medical School São Paulo State University (UNESP)
Department of Chemistry and Biochemistry Biosciences Institute São Paulo State University (UNESP)
Department of Food and Experimental Nutrition Faculty of Pharmaceutical Sciences University of São Paulo (USP)
Department of Morphology Biosciences Institute São Paulo State University (UNESP)
Department Pathology Medical School São Paulo State University (UNESP)
Department of Chemistry and Biochemistry Biosciences Institute São Paulo State University (UNESP)
