Author(s): Goulart, A. J. [UNESP] ; Carmona, E. C. [UNESP] ; Monti, Rubens [UNESP]
Date: 2014
Persistent ID: http://hdl.handle.net/11449/20054
Origin: Oasisbr
Subject(s): alkaline xylanase; Rhizopus stolonifer; solid-state fermentation; alkaline xylanase; alkaline xylanase; Rhizopus stolonifer; Rhizopus stolonifer; solid-state fermentation; solid-state fermentation
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Rhizopus stolonifer was cultivated in wheat bran to produce a cellulase-free alkaline xylanase. The purified enzyme obtained after molecular exclusion chromatography in Sephacryl S-200 HR showed optimum temperature as 45 degrees C and hydrolysis pHs optima as pH 6.0 and 9.0. Xylanase presented higher Vmax at pH 9.0 (0.87 mu mol/mg protein) than at pH 6.0 and minor Km at pH 6.0 (7.42 mg/mL)than at pH 9.0.
Univ Estadual Paulista, Fac Ciências Farmaceut, Dept Alimentos & Nutr, BR-14801902 Araraquara, SP, Brazil
Univ Estadual Paulista, Inst Biociencias, Dept Bioquim & Microbiol, BR-13500900 Rio Claro, SP, Brazil
Univ Estadual Paulista, Fac Ciências Farmaceut, Dept Alimentos & Nutr, BR-14801902 Araraquara, SP, Brazil
Univ Estadual Paulista, Inst Biociencias, Dept Bioquim & Microbiol, BR-13500900 Rio Claro, SP, Brazil
