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Temperature effect on dextransucrase production by Leuconostoc mesenteroides FT 045 B isolated from Alcohol and Sugar Mill Plant

Author(s): Cortezi, M. ; Monti, R. ; Contiero, Jonas [UNESP]

Date: 2014

Persistent ID: http://hdl.handle.net/11449/20055

Origin: Oasisbr

Subject(s): dextran; dextransucrase; Leuconostoc mesenteroides NRRL B512 (F); Leuconostoc mesenteroides FT045 B; temperature; enzyme activity; dextran; dextran; dextransucrase; dextransucrase; Leuconostoc mesenteroides NRRL B512 (F); Leuconostoc mesenteroides NRRL B512 (F); Leuconostoc mesenteroides FT045 B; Leuconostoc mesenteroides FT045 B; temperature; temperature; enzyme activity; enzyme activity


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Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2014-02-26T17:23:58Z No. of bitstreams: 0

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Temperature (23 to 31 degrees C) and sucrose concentration ( 3 and 4%) effects on dextransucrase production by Leuconostoc mesenteroides NRRL B 512 ( F) and Leuconostoc mesenteroides FT 045 B were studied. The conditions in all fermentations were: total reaction volume 2 L, 132 rev. min-1, 0.5 vvm and pH 6.0. The optimum temperature for enzyme yield for strain NRRL B 512 ( F) was 23 degrees C, where at 8-h fermentation was possible to achieve 49.3 DSU/mL. When FT 045 B strain was utilized, 3.2 DSU/mL was obtained at temperature 23 to 25 degrees C.

UNESP, Inst Biociencias Rio Claro, Dept Bioquim & Microbiol, São Paulo, Brazil

UNESP, Fac Ciências Farmaceut, Dept Alimentos & Nutr, São Paulo, Brazil

UNESP, Inst Biociencias Rio Claro, Dept Bioquim & Microbiol, São Paulo, Brazil

UNESP, Fac Ciências Farmaceut, Dept Alimentos & Nutr, São Paulo, Brazil

Document Type Journal article
Language English
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