Author(s): Fernandes, Lu s Gustavo Romani ; Perez-Riverol, Amilcar ; Bazon, Murilo Luiz [UNESP] ; Abram, D bora Moitinho ; Brochetto-Braga, M rcia Regina [UNESP] ; Zollner, Ricardode Lima
Date: 2020
Persistent ID: http://hdl.handle.net/11449/200595
Origin: Oasisbr
Subject(s): CD4+ T cells; Hymenoptera Venom Allergy; Polybia Paulista; CD4+ T cells; CD4+ T cells; Hymenoptera Venom Allergy; Hymenoptera Venom Allergy; Polybia Paulista; Polybia Paulista
Made available in DSpace on 2020-12-12T02:10:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-06-01
Insect venom can cause systemic allergic reactions, including anaphylaxis. Improvements in diagnosis and venom immunotherapy (VIT) are based on a better understanding of an immunological response triggered by venom allergens. Previously, we demonstrated that the recombinant phospholipase A1 (rPoly p 1) from Polybia paulista wasp venom induces specific IgE and IgG antibodies in sensitized mice, which recognized the native allergen. Here, we addressed the T cell immune response of rPoly p 1-sensitized BALB/c mice. Cultures of splenocytes were stimulated with Polybia paulista venom extract and the proliferation of CD8+ and CD4+ T cells and the frequency of T regulatory cells (Tregs) populations were assessed by flow cytometry. Cytokines were quantified in cell culture supernatants in ELISA assays. The in vitro stimulation of T cells from sensitized mice induces a significant proliferation of CD4+ T cells, but not of CD8+ T cells. The cytokine pattern showed a high concentration of IFN- and IL-6, and no significant differences to IL-4, IL-1 and TGF- 1 production. In addition, the rPoly p 1 group showed a pronounced expansion of CD4+CD25+FoxP3+ and CD4+CD25-FoxP3+ Tregs. rPoly p 1 sensitization induces a Th1/Treg profile in CD4+ T cell subset, suggesting its potential use in wasp venom immunotherapy.
Laboratory of Translational Immunology Medicine School FCM University of Campinas
Center of the Study of Social Insects Department of General and Applied Biology Institute of Biosciences of Rio Claro Institute of Biosciences of Rio Claro S o Paulo State University
Laboratory of Arthropods Molecular Biology IBRC UNESP S o Paulo State University
Venoms and Venomous Animal Studies Center CEVAP S o Paulo State University
Laboratory of Arthropods Molecular Biology IBRC UNESP S o Paulo State University
