Author(s): Borsatto, K. C. [UNESP] ; Alevi, K. C.C. [UNESP] ; Oliveira, J. [UNESP] ; Azeredo-Oliveira, M. T.V. [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/200665
Origin: Oasisbr
Subject(s): Cytogenetics; Meiosis; Mexican triatomine; Cytogenetics; Cytogenetics; Meiosis; Meiosis; Mexican triatomine; Mexican triatomine
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
The Triatomini tribe consists of ten genera and is regarded as one of the most important tribes from epidemiological point of view. The genus Dipetalogaster Usinger, 1939 is composed only by the species Dipetalogaster maxima Uhler, 1894. This triatomine is exclusive of the Mexico and is a potential vector for Chagas disease. Besides the epidemiological importance, the insects of the Triatominae subfamily are important biological models for cytogenetic studies. Therefore, in order to contribute to the knowledge on the reproductive biology and assist in citotaxonomy of D. maxima, this study aimed to describe spermatogenesis, as well as confirm the karyotype and heterochromatic patterns of this Mexican triatomine species. The seminiferous tubules were torn, fixed to a cover slip and underwent the cytogenetic technique of Lacto-acetic orcein and C-banding. Through the cytogenetics analysis of testicular material D. maxima it was possible to confirm the karyotype (2n = 22), describe the stages of spermatogenesis and characterize the heterochromatic pattern (restricted to sex chromosome Y) of the species. D. maxima showed the same arrangement of heterochromatin described for Triatoma lecticularia (Stål, 1859) (a species that occur in United States of American and Mexico and is phylogenetically related with D. maxima), highlighting the importance of this analysis as an optimization tool to explore phylogenetic correlations.
Laboratório de Biologia Celular Departamento de Biologia Instituto de Biociências Letras e Ciências Exatas – IBILCE Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESP, Rua Cristóvão Colombo, 2265, Jardim Nazareth
Laboratório de Parasitologia Departamento de Ciências Biológicas Faculdade de Ciências Farmacêuticas – FCFAR Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESP, Rodovia Araraquara-Jaú, Km 1
Laboratório de Biologia Celular Departamento de Biologia Instituto de Biociências Letras e Ciências Exatas – IBILCE Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESP, Rua Cristóvão Colombo, 2265, Jardim Nazareth
Laboratório de Parasitologia Departamento de Ciências Biológicas Faculdade de Ciências Farmacêuticas – FCFAR Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESP, Rodovia Araraquara-Jaú, Km 1
CAPES: 001
