Author(s):
Carvalho, Jaqueline Candido de [UNESP] ; Silva, Fernando Evaristo da [UNESP] ; Rizzoto, Guilherme ; Dadalto, Carmell Rezende [UNESP] ; Rolim, Luna Scarpari [UNESP] ; Mamprim, Maria Jaqueline [UNESP] ; Souza, Fabiana Ferreira de [UNESP] ; Teixeira, Carlos Roberto [UNESP] ; Kastelic, John Patrick ; Ferreira, João Carlos Pinheiro [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/200670
Origin: Oasisbr
Subject(s): Animal reproduction; Male wild canidae; Reproductive ultrasonography; Semen collection; Sperm morphology; Animal reproduction; Animal reproduction; Male wild canidae; Male wild canidae; Reproductive ultrasonography; Reproductive ultrasonography; Semen collection; Semen collection; Sperm morphology; Sperm morphology
Description
Made available in DSpace on 2020-12-12T02:12:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-01
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Our objective was to condition adult male crab-eating fox (Cerdocyon thous) to allow semen collection by digital manipulation of the penis, assess sperm characteristics, and use ultrasonography to assess and measure testes and prostate and determine echotexture, as well as hemodynamics of testicular and spermatic cord arteries. Five captive C. thous (2–5 y, ∼7 kg) were used. After conditioning for digital sperm collection, 13 ejaculates were obtained from two males. Ejaculate characteristics (mean ± SD) were volume (393.2 ± 178.0 μL), concentration (463.7 ± 594.4 × 106 sperm/mL), total sperm number (217.4 ± 304.0 × 106 sperm), total motility (68.0 ± 6.1%), progressive motility (48.8 ± 5.1%) and rapid sperm (57.2 ± 6.8%). The most prevalent sperm morphological defects were abnormal acrosome, small abnormal head, and diadem defect. Under chemical restraint, testes and prostate were evaluated with B-mode and spectral Doppler ultrasonography. Based on ultrasonography, testicular volumes were (2.2 ± 0.7 and 2.1 ± 0.7 cm3 for left and right testes, respectively), whereas prostate dimensions were 2.3 ± 0.4 cm long and 1.5 ± 0.3 cm thick. Lastly, using triplex Doppler, peak systolic velocity (PSV) was higher in testicular versus intratesticular arteries. In conclusion, adult male C. thous were conditioned to allow sperm collection by digital manipulation without urine contamination, enabling evaluation of sperm characteristics. Furthermore, B-mode ultrasound facilitated evaluation of testes and prostate morphometry and echotexture, whereas Doppler was used to characterize testicular blood flow. This was proof of concept for semen collection and baseline data for future male reproductive soundness examinations and development of assisted reproductive techniques in this species.
Department of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)
Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)
Department of Production Animal Health Faculty of Veterinary Medicine University of Calgary
Department of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)
Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)
CAPES: 001
