Author(s):
Mallón, Alina Rodríguez ; González, Luis Javier ; Guzmán, Pedro Enrique Encinosa ; Bechara, Gervasio Henrique [UNESP] ; Sanches, Gustavo Seron [UNESP] ; Pousa, Satomy ; Cabrera, Gleysin ; Cabrales, Ania ; Garay, Hilda ; Mejías, Raúl ; Álvarez, José Raúl López ; Soto, Yamil Bello ; Almeida, Fabiola ; Guirola, Osmany ; Fernández, Rafmary Rodríguez ; Castillo, Alier Fuentes ; Méndez, Luis ; Jiménez, Samanta ; Licea-Navarro, Alexei ; Portela, Madelón ; Durán, Rosario ; Estrada, Mario Pablo
Date: 2020
Persistent ID: http://hdl.handle.net/11449/200678
Origin: Oasisbr
Subject(s): Anti-tick vaccine; Bm86; Chemical conjugation; Cross-linked peptides; P0; Peptide; Anti-tick vaccine; Anti-tick vaccine; Bm86; Bm86; Chemical conjugation; Chemical conjugation; Cross-linked peptides; Cross-linked peptides; P0; P0; Peptide; Peptide
Description
Made available in DSpace on 2020-12-12T02:13:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-06-01
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
A synthetic 20 amino acid peptide of the ribosomal protein P0 from ticks, when conjugated to keyhole limpet hemocyanin from Megathura crenulata and used as an immunogen against Rhipicephalus microplus and Rhipicephalus sanguineus s.l. species, has shown efficacies of around 90%. There is also experimental evidence of a high efficacy of this conjugate against Amblyomma mixtum and Ixodes ricinus species, which suggest that this antigen could be a good broad-spectrum anti-tick vaccine candidate. In this study, the P0 peptide (pP0) was chemically conjugated to Bm86 as a carrier protein. SDS-PAGE analysis of this conjugate demonstrated that it is highly heterogeneous in size, carrying from 1 to 18 molecules of pP0 per molecule of Bm86. Forty-nine out of the 54 lysine residues and the N-terminal end of Bm86 were found partially linked to pP0 by using LC-MS/MS analysis and the combination of four different softwares. Several post-translational modifications of Bm86 protein were also identified by mass spectrometry. High immunogenicity and efficacy were achieved when dogs and cattle were vaccinated with the pP0–Bm86 conjugate and challenged with R. sanguineus s.l. and R. microplus, respectively. These results encourage the development of this antigen with promising possibilities as an anti-tick vaccine.
Animal Biotechnology Department Center for Genetic Engineering and Biotechnology (CIGB)
Mass Spectrometry Laboratory and GlycoLab Department of Proteomics CIGB
Pontifícia Universidade Católica do Paraná (PUCPR)
Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (FCAV-UNESP)
Synthetic Peptides Group CIGB
Instituto de Ciencia Animal (ICA)
Bioinformatic Department CIGB
National Laboratory for Parasitology
Departamento de Innovación Biomédica CICESE
Unidad de Bioquímica y Proteómica Analítica Institut Pasteur de Montevideo
Instituto de Investigaciones Biológicas Clemente Estable (IIBCE)
Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (FCAV-UNESP)
