Author(s):
Dantas, Gabriela N. [UNESP] ; Santarosa, Bianca P. [UNESP] ; Santos, Vitor H. ; Hooper, Henrique B. ; Micai, Ricardo A. ; Sinzato, Yuri K. [UNESP] ; Damasceno, Débora C. [UNESP] ; da Silva, Andreza A. ; Benesi, Fernando J. ; Gonçalves, Roberto C. [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/200707
Origin: Oasisbr
Subject(s): Antioxidant enzymes; Cattle; Lipid peroxidation; Neonatology; Reactive oxygen species; Antioxidant enzymes; Antioxidant enzymes; Cattle; Cattle; Lipid peroxidation; Lipid peroxidation; Neonatology; Neonatology; Reactive oxygen species; Reactive oxygen species
Description
Made available in DSpace on 2020-12-12T02:13:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-08-01
Oxidative stress occurs when there is greater than optimal production of reactive oxygen species (ROS) or an antioxidant system failure. Calves produced using in vitro fertilization (IVF) or cloning (CA) have greater mortality rates, with greater incidence of respiratory diseases, which could be explained by the deleterious outcomes from oxidative stress. Calves were studied that were produced using: artificial insemination (AI; n = 20), in vitro fertilization (IVF; n = 15) or cloning (CA; n = 15). Blood samples were collected at 6, 12, 24 and 48 h subsequent to the time of birth. The cloned calves had greater ROS production from lipid peroxidation, with greater thiobarbituric acid reactive substances. This factor was associated with a lesser amount of superoxide dismutase in the CA. Calves produced using IVF had a greater activity of catalase and glutathione peroxidase, either due to greater production of hydrogen peroxide or greater efficiency of enzymatic response of these neonates. Calves produced using AI had greater concentrations of reduced thiol groups. These associated factors may indicate there is greater oxidative stress in calves produced by IVF and cloning than with use of AI, however in these calves there was an effective response to these oxidative stressors within 48 h subsequent to birth. Hence, calves produced using IVF and by cloning have greater ROS production when compared to calves produced using AI. The calves produced using IVF, however, had a greater enzymatic activity or were more efficient in adapting to ROS when compared to calves produced by cloning.
Department of Veterinary Clinic School of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP)
Department of Veterinary Clinics Agricultural Sciences Center Londrina State University (UEL)
Department of Basic Sciences School of Animal Science and Food Engineering (FZEA) University of São Paulo (USP), Pirassununga Campus
In Vitro Clonagem Animal S/A. Mogi Mirim
Laboratory of Experimental Research on Gynecology and Obstetrics Botucatu Medical School São Paulo State University (UNESP)
Department of Veterinary Medicine and Surgery Federal Rural University of Rio de Janeiro (UFRRJ)
Departament of Medical Clinic (VCM) School of Veterinary Medicine and Animal Science (FMVZ) São Paulo University (USP)
Department of Veterinary Clinic School of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP)
Laboratory of Experimental Research on Gynecology and Obstetrics Botucatu Medical School São Paulo State University (UNESP)
