Author(s):
Cabral-Santos, Carolina [UNESP] ; Silveira, Loreana Sanches ; Chimin, Patricia ; Rosa-Neto, José Cesar ; Lira, Fábio Santos [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/200774
Origin: Oasisbr
Subject(s): Cytokines; Inflammatory response; Lipid metabolism; Peroxisome proliferator activated receptor; Cytokines; Cytokines; Inflammatory response; Inflammatory response; Lipid metabolism; Lipid metabolism; Peroxisome proliferator activated receptor; Peroxisome proliferator activated receptor
Description
Made available in DSpace on 2020-12-12T02:15:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
The nuclear transcriptional factor peroxisome proliferator activated receptor alpha (PPARα) plays a role in regulating genes involved in lipid metabolism, adipogenesis and inflammation. We aimed to assess the role of PPARα on exercise-mediated locally produced cytokines in adipose fat deposits and skeletal muscle. C57BL/6 (WT) and PPARα knockout (PPARα−/−) mice were examined. Each genotype was randomly subdivided into three groups: non-exercised, and euthanized 2 or 24 h after a moderate aerobic exercise session (run on a treadmill at 60% of maximum speed for 1 h). Fat content in gastrocnemius muscle and lipolytic activity in isolated adipose tissue from mesenteric (MEAT) and retroperitoneal (RPAT) adipose tissue were evaluated. In addition, Interleukin 6 (IL-6), interleukin 10 (IL-10), tumor necrosis factor α (TNF-α) and monocyte chemoattractant protein 1 (MCP-1) content were evaluated by ELISA. WT mice showed a maximum lipolysis rate, as well as higher IL-6, IL-10, and IL10/TNF-α ratio values 2 h post-exercise (RPAT only) compared with PPARα−/− mice. Taken together, our data suggests that PPARα knockout mice exhibited reduced lipolysis and anti-inflammatory response in adipose tissue following exercise, PPARα appears to play an important role in immunomodulatory and lipolysis signaling after acute moderate exercise.
Exercise and Immunometabolism Research Group Department of Physical Education Post-Graduation Program in Movement Sciences State University of São Paulo (UNESP) Presidente Prudente
Immunometabolism Research Group Department of Cell and Developmental Biology University of São Paulo
Universidade Estadual de Londrina Centro de Educação Física e Desportos Departamento de Fundamentos da Educação Física
Exercise and Immunometabolism Research Group Department of Physical Education Post-Graduation Program in Movement Sciences State University of São Paulo (UNESP) Presidente Prudente
FAPESP: 2014/01246-6
FAPESP: 2015/26148-0
FAPESP: 2018/17648-7
