Autor(es):
Beserra, Fernando Pereira [UNESP] ; Gushiken, Lucas Fernando Sérgio [UNESP] ; Vieira, Ana Júlia [UNESP] ; Bérgamo, Danilo Augusto [UNESP] ; Bérgamo, Patrícia Luísa [UNESP] ; de Souza, Mariana Oliveira [UNESP] ; Hussni, Carlos Alberto [UNESP] ; Takahira, Regina Kiomi [UNESP] ; Nóbrega, Rafael Henrique [UNESP] ; Martinez, Emanuel Ricardo Monteiro [UNESP] ; Jackson, Christopher John ; Maia, Gabriela Lemos de Azevedo ; Rozza, Ariane Leite [UNESP] ; Pellizzon, Cláudia Helena [UNESP]
Data: 2020
Identificador Persistente: http://hdl.handle.net/11449/200775
Origem: Oasisbr
Assunto(s): Cutaneous wound healing; Lupeol; Natural product; Skin regeneration; Triterpene; Cutaneous wound healing; Cutaneous wound healing; Lupeol; Lupeol; Natural product; Natural product; Skin regeneration; Skin regeneration; Triterpene; Triterpene
Descrição
Made available in DSpace on 2020-12-12T02:15:45Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-07-02
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Skin wound healing is a highly complex event that involves different mediators at the cellular and molecular level. Lupeol has been reported to possess different biological activities, such as anti-inflammatory, antioxidant, antidiabetic, and in vitro wound healing properties, which motivated us to proceed with in vivo studies. We aimed to investigate the wound healing effect of lupeol-based cream for 3, 7, and 14 days. Wound excisions were induced on the thoraco-lumbar region of rats and topically treated immediately after injury induction. Macroscopic, histopathological, and immunohistochemical analyses were performed. Cytokine levels were measured by ELISA and gene expression was evaluated by real-time RT-qPCR. Our results showed a strong wound-healing effect of lupeol-based cream after 7 and 14 days. Lupeol treatment caused a reduction in proinflammatory cytokines (TNF-a, IL-1β, and IL-6) and gene and protein NF-κB expression, and positively altered IL-10 levels, showing anti-inflammatory effects in the three treatment periods. Lupeol treatment showed involvement in the proliferative phase by stimulating the formation of new blood vessels, increasing the immunostaining of Ki-67 and gene expression, and immunolabeling of vascular endothelial growth factor (VEGF) and epidermal growth factor (EGF), and increasing gene expression of transforming growth factor beta-1 (TGF-β1) after seven days of treatment. Lupeol was also involved in the tissue regeneration phase by increasing the synthesis of collagen fibers noted in the three treatment periods analyzed. Our findings suggest that lupeol may serve as a novel therapeutic option to treat cutaneous wounds by regulating mechanisms involved in the inflammatory, proliferative, and tissue-remodeling phases.
Department of Morphology Institute of Biosciences São Paulo State University (UNESP)
Department of Surgery and Veterinary Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)
Department of Clinics Veterinary School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)
Kolling Institute of Medical Research The University of Sydney at Royal North Shore Hospital, St. Leonard
Department of Pharmacy Federal University of São Francisco Valley (UNIVASF)
Department of Morphology Institute of Biosciences São Paulo State University (UNESP)
Department of Surgery and Veterinary Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)
Department of Clinics Veterinary School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)
FAPESP: 2013/23340-1
FAPESP: 2014/23247-4
FAPESP: 2017/17600-1
