Author(s):
Vesentini, Giovana [UNESP] ; Barbosa, Angélica M.P. [UNESP] ; Floriano, Juliana F. [UNESP] ; Felisbino, Sérgio L. [UNESP] ; Costa, Sarah M.B. [UNESP] ; Piculo, Fernanda [UNESP] ; Marini, Gabriela [UNESP] ; Nunes, Sthefanie K. [UNESP] ; Reyes, David R.A. [UNESP] ; Marcondes, João P.C. [UNESP] ; Hallur, Raghavendra L.S. [UNESP] ; Rozza, Ariane L. [UNESP] ; Magalhães, Cláudia G. [UNESP] ; Costa, Roberto [UNESP] ; Abbade, Joelcio F. [UNESP] ; Corrente, José E. [UNESP] ; Calderon, Iracema M.P. [UNESP] ; Matheus, Selma M.M. [UNESP] ; Rudge, Marilza V.C. [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/200818
Origin: Oasisbr
Subject(s): Collagen; Gestational diabetes mellitus; Pregnancy-specific urinary incontinence; Skeletal muscle; Urinary incontinence; Collagen; Collagen; Gestational diabetes mellitus; Gestational diabetes mellitus; Pregnancy-specific urinary incontinence; Pregnancy-specific urinary incontinence; Skeletal muscle; Skeletal muscle; Urinary incontinence; Urinary incontinence
Description
Made available in DSpace on 2020-12-12T02:16:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-08-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Aims: To evaluate the effects of gestational diabetes mellitus (GDM) on the structural characteristics of the rectus abdominis muscle (RAM) and its indirect effects on pregnancy-specific urinary incontinence (PSUI). Methods: A total of 92 pregnant women were divided into four groups, according to their clinical conditions: non-GDM continent, non-GDM associated PSUI, GDM continent and GDM associated PSUI. The muscle morphometry (histochemistry and immunohistochemistry) for the fiber types and collagen fiber distribution, the ultrastructural analysis (transmission electron microscopy), the protein expression of fiber types and calcium signaling (Western blotting), and the content of types I and III collagen fiber (ELISA) in RAM collected at delivery were assessed. Results: The GDM groups presented a significantly increased number of slow fibers and slow-twitch oxidative fiber expression; decreased fiber area, number of fast fibers, and area of collagen; an increase in central nuclei; ultrastructural alterations with focal lesion areas such as myeloid structures, sarcomere disorganization, and mitochondrial alteration. The PSUI groups presented a considerable decrease in types I and III collagen contents and the localization of collagen fiber. Conclusions: Our data reveal that GDM causes morphological, biochemical and physiological changes in the RAM, and this might predispose women to PSUI.
Perinatal Diabetes Research Center University Hospital Botucatu Medical School Univ Estadual Paulista_UNESP
São Paulo State University (UNESP) Botucatu Medical School Department of Gynecology and Obstetrics
São Paulo State University (UNESP) School of Philosophy and Sciences Department of Physical Therapy and Occupational Therapy
São Paulo State University (UNESP) Institute of Biosciences Department of Morphology
Universidade Sagrado Coração Department of Health Sciences
São Paulo State University (UNESP) Institute of Biosciences Biostatistics Department
São Paulo State University (UNESP) Institute of Biosciences Department of Anatomy
Perinatal Diabetes Research Center University Hospital Botucatu Medical School Univ Estadual Paulista_UNESP
São Paulo State University (UNESP) Botucatu Medical School Department of Gynecology and Obstetrics
São Paulo State University (UNESP) School of Philosophy and Sciences Department of Physical Therapy and Occupational Therapy
São Paulo State University (UNESP) Institute of Biosciences Department of Morphology
São Paulo State University (UNESP) Institute of Biosciences Biostatistics Department
São Paulo State University (UNESP) Institute of Biosciences Department of Anatomy
FAPESP: 2014/26852-6
FAPESP: 2016/09710-9
