Author(s):
Bordignon, Sidnei Emilio [UNESP] ; da Silva Delabona, Priscila ; Lima, Deise ; Perrone, Olavo [UNESP] ; da Silva Souza, Márcia Gleice ; Santos, Alberdan Silva ; da Cruz Pradella, José Geraldo ; Boscolo, Maurício [UNESP] ; Gomes, Eleni [UNESP] ; da Silva, Roberto [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/200891
Origin: Oasisbr
Subject(s): Biomass; Cellulase; Filamentous fungi; Lignin; Pretreatment; Biomass; Biomass; Cellulase; Cellulase; Filamentous fungi; Filamentous fungi; Lignin; Lignin; Pretreatment; Pretreatment
Description
Made available in DSpace on 2020-12-12T02:18:49Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-09-01
Liquor from pretreatment of sugarcane bagasse is a potential substrate for multiple purposes due to the high concentration of residual sugars. Nevertheless, several potentially toxic byproducts are also present. However, a few microorganisms are able to overcome this toxicity by growing on these liquors. Twenty-five filamentous fungi were evaluated in submerged cultivation, but none was able to grow using liquor at a concentration of 100% as the liquid medium. However, six fungi were selected for enzyme induction after being grown in diluted liquor at 50% (v/v) using two feed pulses. Induction experiments were performed using 1% untreated and pretreated sugarcane bagasse. FPase and xylanase activities were detected for all six fungi in submerged cultivation, whereas β-glucosidase was observed in four fungi. The highest xylanase activity (28.8 IU mL−1) was at 72 h for T. harzianum P49P11 using pretreated-SCB as an inducer. This work showed a successful alternative for the final destination of liquor residue as substrate for fungi cultivation prior to enzyme production.
Laboratory of Biochemistry and Applied Microbiology São Paulo State University-UNESP IBILCE, 2265 Cristóvão Colombo
Brazilian Bioethanol Science and Technology Laboratory CTBE Polo II de Alta Tecnologia, 10000 Giuseppe Máximo Scolfaro
Laboratory of Systematic Investigation in Biotechnology and Molecular Biodiversity Federal University of Pará (UFPA), Augusto Correa
Laboratory of Biochemistry and Applied Microbiology São Paulo State University-UNESP IBILCE, 2265 Cristóvão Colombo
