Autor(es):
Claudiano, Gustavo S. [UNESP] ; de Moraes, Flávio Ruas [UNESP] ; Fernandes, Dayanne C. [UNESP] ; Vantini, Juliana S. [UNESP] ; Yunis-Aguinaga, Jefferson [UNESP] ; Eto, Silas F. [UNESP] ; Marinho-Neto, Fausto A. [UNESP] ; Macedo, Hugo J. A. ; Manrique, Wilson G. ; Moraes, Julieta R. E. [UNESP]
Data: 2020
Identificador Persistente: http://hdl.handle.net/11449/201009
Origem: Oasisbr
Assunto(s): Aeromonosis; Brain infection; Hemorrhagic septicemia; Inflammation; Aeromonosis; Aeromonosis; Brain infection; Brain infection; Hemorrhagic septicemia; Hemorrhagic septicemia; Inflammation; Inflammation
Descrição
Made available in DSpace on 2020-12-12T02:21:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01
Aeromonosis is an infectious disease of high occurrence in captive fish. It manifests mainly as hemorrhagic septicemia. This study aimed to evaluate the physiopathology manifestations of septic aeromonosis in Piaractus mesopotamicus, considering its clinical manifestations, mortality rates, anatomopathology, and anti-iNOS immunolabeling in the brain. Fish were inoculated in the coelomic cavity with increasing concentrations of A. hydrophila: 1.5 × 108; 1.8 × 108; 2.1 × 108; 2.4 × 108; 2.7 × 108 CF mL−1, histopathology, and anti-iNOS immunolabeling in brain. The animals present erratic swimming (swirling), depigmentation, petechiae, skin suffusions, and high mortality rates up to 100% according to the inoculum concentration. The DL50-96 h was estimated in 1.806 × 108 CFU mL−1. Fish inoculated with 2.1 × 108 CFU mL−1 presented 100% mortality rate. Fish presented neurological signs, congestion, extensive hemorrhage, and glial cells activity confirmed by the anti-iNOS labelling. In addition, the inoculated bacteria was reisolated from the brains. The results demonstrated that aeromonosis physiopathology is multifactorial and the observed neurological signs were caused by the inoculated bacteria. These findings highlight the need for differential diagnosis with other infections that produce encephalitis.
Institute of Biodiversity and Forests Federal University of Western Pará UFOPA
Department of Veterinarian Pathology Faculty of Agrarian and Veterinarian Sciences São Paulo State University Unesp
Department of Technology Faculty of Agrarian and Veterinarian Sciences São Paulo State University Unesp
Aquaculture Center of UNESP, Via Prof. Paulo Donato Castellane, km 05
Veterinary Medicine Course Federal University of Rondonia – UNIR
Department of Veterinarian Pathology Faculty of Agrarian and Veterinarian Sciences São Paulo State University Unesp
Department of Technology Faculty of Agrarian and Veterinarian Sciences São Paulo State University Unesp
Aquaculture Center of UNESP, Via Prof. Paulo Donato Castellane, km 05
